Category: publications

Apr 03 2024

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Filed under publications

Hongtao Liu, Dariusz Zakrzewicz, Kamil Nosol, Rossitza N Irobalieva, Somnath Mukherjee, Rose Bang-Sørensen, Nora Goldmann, Sebastian Kunz, Lorenzo Rossi, Anthony A Kossiakoff, Stephan Urban, Dieter Glebe, Joachim Geyer, Kaspar P Locher

Nature Communications volume 15, Article number: 2476 (2024)

PMID: 38509088 PMCID: PMC10954734 DOI: 10.1038/s41467-024-46706-w

Cellular entry of the hepatitis B and D viruses (HBV/HDV) …

Antibody, antiviral, cryo-EM, cryogenic electron microscopy, drug, Fab, hepatitis, Phage display, Protein structure, virus

Apr 03 2024

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Filed under publications

Szymon P. Kordon, Kristina Cechova, Sumit J. Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A. Kossiakoff, Reza Vafabakhsh, and Demet Araç

bioRxiv 2024 Feb 26

PMID: 38464178 DOI: 10.1101/2024.02.25.581807

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain extracellular regions (ECR) containing a conserved GAIN …

Antibody, cell-adhesion molecules, cryo-EM, cryogenic electron microscopy, Fab, Phage display

Nov 21 2023

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

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Heather A Bruce, Alexander U Singer, Ekaterina V Filippova, Levi L Blazer, Jarrett J Adams, Leonie Enderle, Moshe Ben-David, Elizabeth H Radley, Daniel Y L Mao, Victor Pau, Stephen Orlicky, Frank Sicheri, Igor Kourinov, Shane Atwell, Anthony A Kossiakoff, Sachdev S Sidhu

Protein Sci. 2023 Nov 9:e4824.

PMID: 37945533 DOI: 10.1002/pro.4824

The atomic-resolution structural information …

antibody fragments, antibody library, crystal lattice contacts, crystallization platform, protein engineering, surface entropy reduction, X-ray crystallography

Nov 06 2023

Structural and molecular basis of choline uptake into the brain by FLVCR2

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Rosemary J. Cater, Dibyanti Mukherjee, Eva Gil Iturbe, Satchal K. Erramilli, Ting Chen, Katie Koo, Nicolás Santander Grez, Andrew Reckers, Brian Kloss, Tomasz Gawda, Brendon C. Choy, Zhening Zheng, Oliver B. Clarke, Sook Wah Yee, Anthony A. Kossiakoff, Matthias Quick, Thomas Arnold, Filippo Mancia

bioRxiv. 2023 Oct 5:2023.10.05.561059.

PMID: 37873173 PMCID: PMC10592973 DOI: 10.1101/2023.10.05.561059

Choline is an essential …

cryo-EM, cryogenic electron microscopy, Fab, Protein structure

Oct 17 2023

Structure of human drug transporters OATP1B1 and OATP1B3

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Anca-Denise Ciută, Kamil Nosol, Julia Kowal, Somnath Mukherjee, Ana S. Ramírez, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Nature Communications volume 14, Article number: 5774 (2023)

PMID: 37723174 PMCID: PMC10507018 DOI: 10.1038/s41467-023-41552-8

The organic anion transporting polypeptides OATP1B1 and OATP1B3 are membrane proteins that mediate uptake of drugs into the liver for subsequent conjugation and biliary …

Biochemistry, Fab, Membrane biophysics, Phage display, Protein structure, Structural biology

Oct 17 2023

Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Filed under publications

Paweł P Knejski, Satchal K Erramilli, Anthony A Kossiakoff

bioRxiv. 2023 Aug 1:2023.08.01.551527.

PMID: 37577460 PMCID: PMC10418163 DOI: 10.1101/2023.08.01.551527

Pathogenic bacteria, such as Pseudomonas aeruginosa , depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding …

cryo-EM, cryogenic electron microscopy, Fab, Fiducial markers, Phage display, Protein structure

Aug 08 2023

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Filed under publications

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

Antibody, cryo-EM, cryogenic electron microscopy, Fab, membrane protein, Phage display, Protein structure

Aug 08 2023

ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

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Qiuyan Chen, Christopher T Schafer, Somnath Mukherjee, Martin Gustavsson, Parth Agrawal, Xin-Qiu Yao, Anthony A Kossiakoff, Tracy M Handel, John J G Tesmer

bioRxiv 2023 Jul 19, 2023

PMID: 37502840 PMCID: PMC10370059 DOI: 10.1101/2023.07.18.549504

Atypical chemokine receptor 3 (ACKR3, also known as CXCR7) is a scavenger receptor that regulates extracellular levels of the chemokine CXCL12 …

Antibody, cryo-EM, cryogenic electron microscopy, Phage display, Protein structure

Aug 03 2023

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Filed under publications

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2023 Jun 12, 2023

PMID: 37398044 PMCID: PMC10312657 DOI: 10.1101/2023.06.12.544689

Claudins are a family of ∼25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. Humans have 27 subtypes, which homo- and hetero-oligomerize to …

cryo-EM, cryogenic electron microscopy, membrane protein, Phage display, Protein structure

Jul 24 2023

Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Filed under publications

Satchal K. Erramilli, Pawel K. Dominik, Dawid Deneka , Piotr Tokarz , Sangwoo S. Kim, Bharat G. Reddy, Blazej M. Skrobek , Olivier Dalmas , Eduardo Perozo, Anthony A. Kossiakoff

J Mol Biol. 2023

PMD: 37394032 PMID: 37394032 DOI: 10.1016/j.jmb.2023.168192

CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg2+, and highly asymmetric …

Antibody, CorA, ion channel, membrane protein, Phage display, synthetic antibodies, X-ray crystallography

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Category: publications

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

Structural and molecular basis of choline uptake into the brain by FLVCR2

Structure of human drug transporters OATP1B1 and OATP1B3

Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

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2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies