Tag: Antibody

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

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ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Qiuyan Chen, Christopher T Schafer, Somnath Mukherjee, Martin Gustavsson, Parth Agrawal, Xin-Qiu Yao, Anthony A Kossiakoff, Tracy M Handel, John J G Tesmer

bioRxiv 2023 Jul 19, 2023

PMID: 37502840 PMCID: PMC10370059 DOI: 10.1101/2023.07.18.549504

Atypical chemokine receptor 3 (ACKR3, also known as CXCR7) is a scavenger receptor that regulates extracellular levels of the chemokine CXCL12 …

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Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Satchal K. Erramilli, Pawel K. Dominik, Dawid Deneka , Piotr Tokarz , Sangwoo S. Kim, Bharat G. Reddy, Blazej M. Skrobek , Olivier Dalmas , Eduardo Perozo, Anthony A. Kossiakoff 

J Mol Biol. 2023

PMD: 37394032 PMID: 37394032 DOI: 10.1016/j.jmb.2023.168192

CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg2+, and highly asymmetric …

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Structural basis for assembly and lipid-mediated gating of LRRC8A: C volume-regulated anion channels

David M Kern, Julia Bleier, Somnath Mukherjee, Jennifer M Hill, Anthony A Kossiakoff, Ehud Y Isacoff, Stephen G Brohawn

Nat Struct Mol Biol 2023 Mar 16

PMID: 36928458 DOI: 10.1038/s41594-023-00944-6

Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate cells as …

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Architecture of the cytoplasmic face of the nuclear pore

Christopher J Bley, Si Nie, George W Mobbs, Stefan Petrovic, Anna T Gres, Xiaoyu Liu, Somnath Mukherjee, Sho Harvey, Ferdinand M Huber, Daniel H Lin, Bonnie Brown, Aaron W Tang, Emily J Rundlet, Ana R Correia, Shane Chen, Saroj G Regmi, Taylor A Stevens, Claudia A Jette, Mary Dasso, Alina Patke, Alexander F Palazzo, Anthony …

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Protein Engineering: Advances in Phage Display for Basic Science and Medical Research

Elena K Davydova

Review Biochemistry (Mosc). 2022 Jan;87(Suppl 1):S146-S110

PMID: 35501993 PMCID: PMC8802281 DOI: 10.1134/S0006297922140127

Functional Protein Engineering became the hallmark of biomolecule manipulation in the new millennium, building on and surpassing the underlying structural DNA manipulation and recombination techniques developed and employed in the last decades of the 20th century. Because of their prominence …

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)

PMID: 34782475 DOI: 10.1073/pnas.2115435118

With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …

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A T cell redirection platform for co-targeting dual antigens on solid tumors

Leonie Enderle, Karim H Shalaby, Maryna Gorelik, Alexander Weiss, Levi L Blazer, Marcin Paduch, Lia Cardarelli, Anthony Kossiakoff, Jarrett J Adams, Sachdev S Sidhu

MAbs. Jan-Dec 2021;13(1):1933690. doi: 10.1080/19420862.2021.1933690

PMID: 34190031

In order to direct T cells to specific features of solid cancer cells, we engineered a bispecific antibody format, named Dual Antigen T cell …

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