Kossiakoff Lab – University of Chicago

Oct 14 2024

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Filed under publications

Kadidia Samassekou, Elisabeth E. Garland-Kuntz, Vaani Ohri, Isaac J. Fisher, Satchal K. Erramilli,Kaushik Muralidharan, Livia M. Bogdan, Abigail M. Gick, Anthony A. Kossiakoff, Angeline M. Lyon

bioRxiv 2024 September 12

PMID: 39314324 PMCID: PMC11418990 DOI: 10.1101/2024.09.11.612521

Phospholipase Cε (PLCε) increases intracellular Ca 2+ and protein kinase C (PKC) activity in the cardiovascular system in response …

cryo-EM, cryogenic electron microscopy, Fab, Phage display

Oct 14 2024

Structural insights into translocation and tailored synthesis of hyaluronan

Filed under publications

Ireneusz Górniak, Zachery Stephens, Satchal K. Erramilli, Tomasz Gawda, Anthony A. Kossiakoff & Jochen Zimmer

Nature Structural & Molecular Biology (2024)

PMID: 39322765 DOI: 10.1038/s41594-024-01389-1

Hyaluronan (HA) is an essential component of the vertebrate extracellular matrix. It is a heteropolysaccharide of N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcA) reaching several megadaltons in healthy tissues. HA is …

cryo-EM, cryogenic electron microscopy, Fab, Glycobiology, Phage display

Oct 14 2024

Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Filed under publications

Yaqi Liu, Chelsea M Brown, Nuno Borges, Rodrigo N Nobre, Satchal Erramilli, Meagan Belcher Dufrisne, Brian Kloss, Sabrina Giacometti, Ana M Esteves, Cristina G Timoteo, Piotr Tokarz, Rosemary Cater, Yasu S Morita, Anthony A Kossiakoff, Helena Santos, Phillip J Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 18

PMID: 39345506 PMCID: PMC11429742 DOI: 10.1101/2024.09.17.613550

Tuberculosis …

cryo-EM, cryogenic electron microscopy, Fab, Phage display, Protein structure, Structural biology

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Structural insights into translocation and tailored synthesis of hyaluronan

Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Engineered Protein-G variants for plug-and-play applications

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Structural insights into translocation and tailored synthesis of hyaluronan

Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

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2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies