Kossiakoff Lab – University of Chicago

Jan 28 2025

Engineered protein G variants for multifunctional antibody-based assemblies

Filed under publications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

Protein Science 2025 Feb

PMID: 39865354 DOI: 10.1002/pro.70019

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from multiple pairs of …

antibody engineering, antibody engineering; bispecific antibodies; cell biology applications; engineered protein G modular protein assembly, Bispecific antibodies, cell biology applications, engineered protein G, modular protein assembly

Jan 02 2025

Molecular characterization of the archaic HLA-B*73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2

Filed under publications

Philipp Ross, Hugo G. Hilton, Jane Lodwick, Tomasz Slezak, Lisbeth A. Guethlein, Curtis P. McMurtrey, Alex S. Han, Morten Nielsen, Daniel Yong, Charles L. Dulberger, Kristof T. Nolan, Sobhan Roy, Caitlin D. Castro, William H. Hildebrand, Minglei Zhao, Anthony Kossiakoff, Peter Parham, Erin J. Adams

bioRxiv 2024 Nov 28:2024.11.25.625330.

PMID: 39651149 PMCID: PMC11623575 DOI: 10.1101/2024.11.25.625330

…

cryogenic electron microscopy, Evolutionary Biology, X-ray crystallography

Jan 02 2025

Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function

Filed under publications

Szymon P Kordon, Kristina Cechova, Sumit J Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A Kossiakoff, Reza Vafabakhsh, Demet Araç

Nature Communications 2024 Dec 4;15:10545.

PMID: 39627215 PMCID: PMC11615224 DOI: 10.1038/s41467-024-54836-4

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain extracellular regions (ECRs) containing a …

Cell signalling, cryogenic electron microscopy, G protein-coupled receptors, Single-molecule biophysics

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Engineered protein G variants for multifunctional antibody-based assemblies

Molecular characterization of the archaic HLA-B*73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2

Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function

Biophysical Basis of Paracellular Barrier Modulation by a Pan-Claudin-Binding Molecule

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Engineered protein G variants for multifunctional antibody-based assemblies

Molecular characterization of the archaic HLA-B*73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2

Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function

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Automated antibody phage display selection and screening system

Recent News

2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies