An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability

Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA

Protein Sci. 2019 Oct;

PMID: 31622515

Abstract

Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to …

Continue reading

Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin

Farcasanu M, Wang AG, Uchański T, Bailey LJ, Yue J, Chen Z, Wu X, Kossiakoff A, Tang WJ

Biochemistry 2019 Jun;

PMID: 31243996

Abstract

Anthrax, a lethal, weaponizable disease caused by Bacillus anthracis, acts through exotoxins that are primary mediators of systemic toxicity and also targets for neutralization by passive immunotherapy. The ease of engineering …

Continue reading

Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2

Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S

Proc. Natl. Acad. Sci. U.S.A. 2018 Sep;

PMID: 30224453

Abstract

The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational modifications associated with …

Continue reading