Engineered ultra-high affinity synthetic antibodies for SARS-CoV-2 neutralization and detection

Slezak T, Kossiakoff AA

J Mol Biol. 2021 Mar 25;doi: 10.1016/j.jmb.2021.166956.

PMID: 33775667

Abstract

The Covid-19 pandemic is a centenarial global catastrophe. Similar events are likely to be recurring with more frequency in the future. The inability to control the virus’ impact is caused by many factors, but the lack of a technology infrastructure to …

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Recognition of an α-helical hairpin in P22 large terminase by a synthetic antibody fragment

Lokareddy RK, Ko YH, Hong N, Doll SG, Paduch M, Niederweis M, Kossiakoff AA, Cingolani G

Acta Crystallogr D Struct Biol. 2020 Sep 1;76(Pt 9):876-888.

PMID: 32876063

Abstract

The genome-packaging motor of tailed bacteriophages and herpesviruses is a multisubunit protein complex formed by several copies of a large (TerL) and a small (TerS) terminase subunit. …

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Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC.

Elife. 2020 Aug 7;9:e58464.

PMID: 32762848

Abstract

Frizzleds (Fzd) are the primary receptors for Wnt morphogens, which are essential regulators of stem cell biology, yet the structural basis of Wnt signaling through Fzd remains …

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