Kossiakoff Lab – University of Chicago

Aug 12 2024

Engineered Protein-G variants for plug-and-play applications

Filed under publications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

bioRxiv 2024 August 08.

DOI: 10.1101/2024.08.06.606809

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from …

Bispecific antibodies, Fabs

Aug 07 2024

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Filed under publications

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which contains several minor vault components (MVCs) and …

cryo-EM, cryogenic electron microscopy, Phage display, Protein structure, sAB

Aug 07 2024

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Filed under publications

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2024 Jul 12:2024.07.11.603128. doi: 10.1101/2024.07.11.603128.

PMID: 39026804 PMCID: PMC11257603 DOI: 10.1101/2024.07.11.603128

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE binds selectively to membrane protein receptors called …

cryogenic electron microscopy, Protein structure, X-ray crystallography

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Engineered Protein-G variants for plug-and-play applications

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Development of Luminescent Biosensors for Calprotectin

Engineered Protein-G variants for plug-and-play applications

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

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Automated antibody phage display selection and screening system

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2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies