Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Karthik Srinivasan, Satchal K. Erramilli, Srinivas Chakravarthy, Adrian Gonzalez, Anthony Kossiakoff, Nicholas Noinaj

Structure. 2023 Mar 17; S0969-2126(23)00078-3.

PMID: 36977410 DOI: 10.1016/j.str.2023.03.002

Roughly 95% of the proteins that make up the chloroplast must be imported from the cytoplasm. The machinery responsible for the translocation of these cargo proteins is called the translocon at the outer …

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Structural basis for assembly and lipid-mediated gating of LRRC8A: C volume-regulated anion channels

David M Kern, Julia Bleier, Somnath Mukherjee, Jennifer M Hill, Anthony A Kossiakoff, Ehud Y Isacoff, Stephen G Brohawn

Nat Struct Mol Biol 2023 Mar 16

PMID: 36928458 DOI: 10.1038/s41594-023-00944-6

Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate cells as …

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Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder

Szymon P Kordon, Przemysław Dutka, Justyna M Adamska, Sumit J Bandekar, Katherine Leon, Satchal K Erramilli, Brock Adams, Jingxian Li, Anthony A Kossiakoff, Demet Araç

Nat Commun. 2023 Feb 6

PMID: 36746957 PMCID: PMC9902482 DOI: 10.1038/s41467-023-36312-7

Adhesion G protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple ligands to regulate …

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