Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins

Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA

Nat Commun 2020 Mar;11(1):1598

PMID: 32221310

Abstract

We propose the concept of universal fiducials based on a set of pre-made …

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Structure and mechanism of the ER-based glucosyltransferase ALG6

Bloch JS, Pesciullesi G, Boilevin J, Nosol K, Irobalieva RN, Darbre T, Aebi M, Kossiakoff AA, Reymond JL, Locher KP

Nature 2020 03;579(7799):443-447

PMID: 32103179

Abstract

In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins. The final seven steps occur in the lumen …

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Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F

Nature 2019 Nov;

PMID: 31776516

Abstract

The emergence and spread of drug-resistant Plasmodium falciparum impedes global efforts to control …

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