Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F

Nature 2019 Nov;

PMID: 31776516

Abstract

The emergence and spread of drug-resistant Plasmodium falciparum impedes global efforts to control …

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Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM

Dutka P, Mukherjee S, Gao X, Kang Y, de Waal PW, Wang L, Zhuang Y, Melcher K, Zhang C, Xu HE, Kossiakoff AA

Structure 2019 Oct;

PMID: 31669042

Abstract

“Universal” synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling …

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An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability

Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA

Protein Sci. 2019 Oct;

PMID: 31622515

Abstract

Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to …

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