Tag: Protein structure

Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Yaqi Liu, Chelsea M Brown, Nuno Borges, Rodrigo N Nobre, Satchal Erramilli, Meagan Belcher Dufrisne, Brian Kloss, Sabrina Giacometti, Ana M Esteves, Cristina G Timoteo, Piotr Tokarz, Rosemary Cater, Yasu S Morita, Anthony A Kossiakoff, Helena Santos, Phillip J Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 18

PMID: 39345506 PMCID: PMC11429742 DOI: 10.1101/2024.09.17.613550

Tuberculosis …

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Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Yaqi Liu, Chelsea M. Brown, Satchal Erramilli, Yi-Chia Su, Po-Sen Tseng, Yu-Jen Wang, Nam Ha Duong, Piotr Tokarz, Brian Kloss, Cheng-Ruei Han, Hung-Yu Chen, José Rodrigues, Margarida Archer, Todd L. Lowary, Anthony A. Kossiakoff, Phillip J. Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 17

PMID: 39345558 PMCID: PMC11429727 DOI: 10.1101/2024.09.17.613533

The emergence of drug-resistant …

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Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which contains several minor vault components (MVCs) and …

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Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

Science Direct Structure 8 October 2024

PMID: 39383874 DOI:10.1016/j.str.2024.09.015

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE binds selectively to membrane protein receptors called claudins on …

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Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial …

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Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Hongtao Liu, Dariusz Zakrzewicz, Kamil Nosol, Rossitza N Irobalieva, Somnath Mukherjee, Rose Bang-Sørensen, Nora Goldmann, Sebastian Kunz, Lorenzo Rossi, Anthony A Kossiakoff, Stephan Urban, Dieter Glebe, Joachim Geyer, Kaspar P Locher

Nature Communications volume 15, Article number: 2476 (2024)

PMID: 38509088 PMCID: PMC10954734 DOI: 10.1038/s41467-024-46706-w

Cellular entry of the hepatitis B and D viruses (HBV/HDV) …

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Structural and molecular basis of choline uptake into the brain by FLVCR2

Rosemary J. Cater, Dibyanti Mukherjee, Eva Gil Iturbe, Satchal K. Erramilli, Ting Chen, Katie Koo, Nicolás Santander Grez, Andrew Reckers, Brian Kloss, Tomasz Gawda, Brendon C. Choy, Zhening Zheng, Oliver B. Clarke, Sook Wah Yee, Anthony A. Kossiakoff, Matthias Quick, Thomas Arnold, Filippo Mancia

bioRxiv. 2023 Oct 5:2023.10.05.561059.

PMID: 37873173 PMCID: PMC10592973 DOI: 10.1101/2023.10.05.561059

Choline is an essential …

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Structure of human drug transporters OATP1B1 and OATP1B3

Anca-Denise Ciută, Kamil Nosol, Julia Kowal, Somnath Mukherjee, Ana S. Ramírez, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Nature Communications volume 14, Article number: 5774 (2023)

PMID: 37723174 PMCID: PMC10507018 DOI: 10.1038/s41467-023-41552-8

The organic anion transporting polypeptides OATP1B1 and OATP1B3 are membrane proteins that mediate uptake of drugs into the liver for subsequent conjugation and biliary …

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Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Paweł P Knejski, Satchal K Erramilli, Anthony A Kossiakoff

bioRxiv. 2023 Aug 1:2023.08.01.551527.

PMID: 37577460 PMCID: PMC10418163 DOI: 10.1101/2023.08.01.551527

Pathogenic bacteria, such as Pseudomonas aeruginosa , depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding …

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Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

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