Tag: cryo-EM

Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias

Yu-Chen Yen, Christopher T Schafer, Martin Gustavsson, Stefanie A Eberle, Pawel K Dominik, Dawid Deneka, Penglie Zhang, Thomas J Schall, Anthony A Kossiakoff, John J G Tesmer, Tracy M Handel

Sci Adv. 2022 Jul 15;8(28):eabn8063.

PMID: 35857509 PMCID: PMC9278869 DOI: 10.1126/sciadv.abn8063

Both CXC chemokine receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) are activated by …

Continue reading

A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel

Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport

Nature. 2022 Jun 29

PMID: 35768507 DOI: 10.1038/s41586-022-04903-x

Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then …

Continue reading

Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver

Hongtao Liu, Rossitza N. Irobalieva, Rose Bang-Sørensen, Kamil Nosol, Somnath Mukherjee, Parth Agrawal, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Cell Research (2022) 0:1–4

PMID: 35726088 DOI: 10.1038/s41422-022-00680-4

The sodium taurocholate (TC) co-transporting polypeptide NTCP (SLC10A1) is a secondary active membrane transport protein that mediates the uptake of bile salts from the portal blood plasma …

Continue reading

Architecture of the cytoplasmic face of the nuclear pore

Christopher J Bley, Si Nie, George W Mobbs, Stefan Petrovic, Anna T Gres, Xiaoyu Liu, Somnath Mukherjee, Sho Harvey, Ferdinand M Huber, Daniel H Lin, Bonnie Brown, Aaron W Tang, Emily J Rundlet, Ana R Correia, Shane Chen, Saroj G Regmi, Taylor A Stevens, Claudia A Jette, Mary Dasso, Alina Patke, Alexander F Palazzo, Anthony …

Continue reading

Structural basis of lipopolysaccharide maturation by the O-antigen ligase

Khuram U. Ashraf, Rie Nygaard, Owen N. Vickery, Satchal K. Erramilli, Carmen M. Herrera, Thomas H. McConville, Vasileios I. Petrou, Sabrina I. Giacometti, Meagan Belcher Dufrisne, Kamil Nosol, Allen P. Zinkle, Chris L. B. Graham, Michael Loukeris, Brian Kloss, Karolina Skorupinska-Tudek, Ewa Swiezewska, David I. Roper, Oliver B. Clarke, Anne-Catrin Uhlemann, Anthony A. Kossiakoff, M. …

Continue reading

Structures of ABCB4 provide insight into phosphatidylcholine translocation

Kamil Nosol, Rose Bang-Sørensen, Rossitza N Irobalieva, Satchal K Erramilli, Bruno Stieger, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci USA 2021 Aug 17;118(33):e2106702118. 

PMID: 34385322 PMCID: PMC8379956 (available on 2022-02-12)

DOI: 10.1073/pnas.2106702118

ABCB4 is expressed in hepatocytes and translocates phosphatidylcholine into bile canaliculi. The mechanism of specific lipid recruitment from the canalicular …

Continue reading