Tag: cryo-EM

Structural and molecular basis of choline uptake into the brain by FLVCR2

Rosemary J. Cater, Dibyanti Mukherjee, Eva Gil Iturbe, Satchal K. Erramilli, Ting Chen, Katie Koo, Nicolás Santander Grez, Andrew Reckers, Brian Kloss, Tomasz Gawda, Brendon C. Choy, Zhening Zheng, Oliver B. Clarke, Sook Wah Yee, Anthony A. Kossiakoff, Matthias Quick, Thomas Arnold, Filippo Mancia

bioRxiv. 2023 Oct 5:2023.10.05.561059.

PMID: 37873173 PMCID: PMC10592973 DOI: 10.1101/2023.10.05.561059

Choline is an essential …

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Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Paweł P Knejski, Satchal K Erramilli, Anthony A Kossiakoff

bioRxiv. 2023 Aug 1:2023.08.01.551527.

PMID: 37577460 PMCID: PMC10418163 DOI: 10.1101/2023.08.01.551527

Pathogenic bacteria, such as Pseudomonas aeruginosa , depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding …

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Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

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ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Qiuyan Chen, Christopher T Schafer, Somnath Mukherjee, Martin Gustavsson, Parth Agrawal, Xin-Qiu Yao, Anthony A Kossiakoff, Tracy M Handel, John J G Tesmer

bioRxiv 2023 Jul 19, 2023

PMID: 37502840 PMCID: PMC10370059 DOI: 10.1101/2023.07.18.549504

Atypical chemokine receptor 3 (ACKR3, also known as CXCR7) is a scavenger receptor that regulates extracellular levels of the chemokine CXCL12 …

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Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2023 Jun 12, 2023

PMID: 37398044 PMCID: PMC10312657 DOI: 10.1101/2023.06.12.544689

Claudins are a family of ∼25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. Humans have 27 subtypes, which homo- and hetero-oligomerize to …

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Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Karthik Srinivasan, Satchal K. Erramilli, Srinivas Chakravarthy, Adrian Gonzalez, Anthony Kossiakoff, Nicholas Noinaj

Structure. 2023 Mar 17; S0969-2126(23)00078-3.

PMID: 36977410 DOI: 10.1016/j.str.2023.03.002

Roughly 95% of the proteins that make up the chloroplast must be imported from the cytoplasm. The machinery responsible for the translocation of these cargo proteins is called the translocon at the outer …

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Structural basis for assembly and lipid-mediated gating of LRRC8A: C volume-regulated anion channels

David M Kern, Julia Bleier, Somnath Mukherjee, Jennifer M Hill, Anthony A Kossiakoff, Ehud Y Isacoff, Stephen G Brohawn

Nat Struct Mol Biol 2023 Mar 16

PMID: 36928458 DOI: 10.1038/s41594-023-00944-6

Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate cells as …

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Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias

Yu-Chen Yen, Christopher T Schafer, Martin Gustavsson, Stefanie A Eberle, Pawel K Dominik, Dawid Deneka, Penglie Zhang, Thomas J Schall, Anthony A Kossiakoff, John J G Tesmer, Tracy M Handel

Sci Adv. 2022 Jul 15;8(28):eabn8063.

PMID: 35857509 PMCID: PMC9278869 DOI: 10.1126/sciadv.abn8063

Both CXC chemokine receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) are activated by …

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A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel

Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport

Nature. 2022 Jun 29

PMID: 35768507 DOI: 10.1038/s41586-022-04903-x

Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then …

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Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver

Hongtao Liu, Rossitza N. Irobalieva, Rose Bang-Sørensen, Kamil Nosol, Somnath Mukherjee, Parth Agrawal, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Cell Research (2022) 0:1–4

PMID: 35726088 DOI: 10.1038/s41422-022-00680-4

The sodium taurocholate (TC) co-transporting polypeptide NTCP (SLC10A1) is a secondary active membrane transport protein that mediates the uptake of bile salts from the portal blood plasma …

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