Category: publications

Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo

Mateja A, Paduch M, Chang HY, Szydlowska A, Kossiakoff AA, Hegde RS, Keenan RJ

Science 2015 Mar;347(6226):1152-5

PMID: 25745174

Abstract

Tail-anchored (TA) proteins are a physiologically important class of membrane proteins targeted to the endoplasmic reticulum by the conserved guided-entry of TA proteins (GET) pathway. During transit, their hydrophobic transmembrane domains (TMDs) are chaperoned by …

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Scalable high throughput selection from phage-displayed synthetic antibody libraries

Miersch S, Li Z, Hanna R, McLaughlin ME, Hornsby M, Matsuguchi T, Paduch M, Sääf A, Wells J, Koide S, Kossiakoff A, Sidhu SS

J Vis Exp 2015 Jan;(95):51492

PMID: 25651360

Abstract

The demand for antibodies that fulfill the needs of both basic and clinical research applications is high and will dramatically increase in the …

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Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling

Rizk SS, Kouadio JL, Szymborska A, Duguid EM, Mukherjee S, Zheng J, Clevenger CV, Kossiakoff AA

Cell Commun. Signal 2015 Jan;13:1

PMID: 25589173

Abstract

BACKGROUND: Many receptors function by binding to multiple ligands, each eliciting a distinct biological output. The extracellular domain of the human prolactin receptor (hPRL-R) uses an identical epitope to bind to …

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Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments

Bailey LJ, Sheehy KM, Hoey RJ, Schaefer ZP, Ura M, Kossiakoff AA

J. Immunol. Methods 2014 Dec;415:24-30

PMID: 25450256

Abstract

Immunoglobulin binding proteins (IBPs) are broadly used as reagents for the purification and detection of antibodies. Among the IBPs, the most widely used are Protein-A and Protein-G. The C2 domain of Protein-G from Streptococcus is …

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A synthetic antibody fragment targeting nicastrin affects assembly and trafficking of γ-secretase

Zhang X, Hoey R, Koide A, Dolios G, Paduch M, Nguyen P, Wu X, Li Y, Wagner SL, Wang R, Koide S, Sisodia SS

J. Biol. Chem. 2014 Dec;289(50):34851-61

PMID: 25352592

Abstract

The γ-secretase complex, composed of presenilin, nicastrin (NCT), anterior pharynx-defective 1 (APH-1), and presenilin enhancer 2 (PEN-2), is assembled in a highly regulated …

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Visualization of arrestin recruitment by a G-protein-coupled receptor

Shukla AK, Westfield GH, Xiao K, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu SS, Koide S, Penczek PA, Kossiakoff AA, Woods VL, Kobilka BK, Skiniotis …

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Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies

Welch BD, Paduch M, Leser GP, Bergman Z, Kors CA, Paterson RG, Jardetzky TS, Kossiakoff AA, Lamb RA

J. Virol. 2014 Oct;88(20):11713-25

PMID: 25122782

Abstract

Paramyxoviruses are enveloped negative-strand RNA viruses that are significant human and animal pathogens. Most paramyxoviruses infect host cells via the concerted action of a tetrameric attachment protein (variously called HN, …

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Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Cell Rep 2014 Jul;8(1):297-310

PMID: …

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Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain

Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E

Nat. Struct. Mol. Biol. 2014 Mar;21(3):244-52

PMID: 24487958

Abstract

The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing …

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New Tricks for an Old Dimer

Wells JA, Kossiakoff AA

Cell Biology, Volume 344, Issue 6185, pp. 703-704

Abstract

Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …

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