Tag: cryogenic electron microscopy

Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes

Benjamin J. Orlando, Pawel K. Dominik, Sourav Roy, Chinemerem P. Ogbu, Satchal K. Erramilli, AnthonyA. Kossiakoff, Alex J. Vecchio

JBC Journal of Biological Chemistry 9 August 2022, 102357

DOI: jbc.2022.102357

Strains of Clostridium perfringens produce a two-domain enterotoxin (CpE) that afflicts humans and domesticated animals, causing prevalent gastrointestinal illnesses. CpE’s C-terminal domain (cCpE) binds cell …

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)

PMID: 34782475 DOI: 10.1073/pnas.2115435118

With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …

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