Tag: Phage display

Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Karthik Srinivasan, Satchal K. Erramilli, Srinivas Chakravarthy, Adrian Gonzalez, Anthony Kossiakoff, Nicholas Noinaj

Structure. 2023 Mar 17; S0969-2126(23)00078-3.

PMID: 36977410 DOI: 10.1016/j.str.2023.03.002

Roughly 95% of the proteins that make up the chloroplast must be imported from the cytoplasm. The machinery responsible for the translocation of these cargo proteins is called the translocon at the outer …

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Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder

Szymon P Kordon, Przemysław Dutka, Justyna M Adamska, Sumit J Bandekar, Katherine Leon, Satchal K Erramilli, Brock Adams, Jingxian Li, Anthony A Kossiakoff, Demet Araç

Nat Commun. 2023 Feb 6

PMID: 36746957 PMCID: PMC9902482 DOI: 10.1038/s41467-023-36312-7

Adhesion G protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple ligands to regulate …

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A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel

Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport

Nature. 2022 Jun 29

PMID: 35768507 DOI: 10.1038/s41586-022-04903-x

Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then …

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Architecture of the cytoplasmic face of the nuclear pore

Christopher J Bley, Si Nie, George W Mobbs, Stefan Petrovic, Anna T Gres, Xiaoyu Liu, Somnath Mukherjee, Sho Harvey, Ferdinand M Huber, Daniel H Lin, Bonnie Brown, Aaron W Tang, Emily J Rundlet, Ana R Correia, Shane Chen, Saroj G Regmi, Taylor A Stevens, Claudia A Jette, Mary Dasso, Alina Patke, Alexander F Palazzo, Anthony …

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Quaternary structure independent folding of voltage-gated ion channel pore domain subunits

Cristina Arrigoni, Marco Lolicato, David Shaya, Ahmed Rohaim, Felix Findeisen, Lam-Kiu Fong, Claire M Colleran, Pawel Dominik, Sangwoo S Kim, Jonathan P Schuermann, William F DeGrado, Michael Grabe, Anthony A Kossiakoff, Daniel L Minor

Nat Struct Mol Biol. 2022 Jun;29(6):537-548.

PMID: 35655098 DOI: 10.1038/s41594-022-00775-x

Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from …

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Synthetic antibody binders detect distinct cellular states of Chromosome Passenger Complex proteins

Marcin Ura, Somnath Mukherjee, Edyta Marcon, Stefan A Koestler, Anthony A Kossiakoff

J Mol Biol. 2022 Apr 22;167602.

PMID: 35469831 DOI: 10.1016/j.jmb.2022.167602

High-performance affinity reagents are essential tools to enable biologists to profile the cellular location and composition of macromolecular complexes undergoing dynamic reorganization. To support further development of such tools, we have assembled a …

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K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle

Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff, Lei Liu & Minglei Zhao

Nat Chem Biol. 2021 Jul 8. doi: 10.1038/s41589-021-00823-5

PMID: 34239127

Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin via different linkages. Deciphering the cellular ubiquitin …

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