Tag: Phage display

Structure of human drug transporters OATP1B1 and OATP1B3

Anca-Denise Ciută, Kamil Nosol, Julia Kowal, Somnath Mukherjee, Ana S. Ramírez, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Nature Communications volume 14, Article number: 5774 (2023)

PMID: 37723174 PMCID: PMC10507018 DOI: 10.1038/s41467-023-41552-8

The organic anion transporting polypeptides OATP1B1 and OATP1B3 are membrane proteins that mediate uptake of drugs into the liver for subsequent conjugation and biliary …

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Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Paweł P Knejski, Satchal K Erramilli, Anthony A Kossiakoff

bioRxiv. 2023 Aug 1:2023.08.01.551527.

PMID: 37577460 PMCID: PMC10418163 DOI: 10.1101/2023.08.01.551527

Pathogenic bacteria, such as Pseudomonas aeruginosa , depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding …

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Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

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ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Qiuyan Chen, Christopher T Schafer, Somnath Mukherjee, Martin Gustavsson, Parth Agrawal, Xin-Qiu Yao, Anthony A Kossiakoff, Tracy M Handel, John J G Tesmer

bioRxiv 2023 Jul 19, 2023

PMID: 37502840 PMCID: PMC10370059 DOI: 10.1101/2023.07.18.549504

Atypical chemokine receptor 3 (ACKR3, also known as CXCR7) is a scavenger receptor that regulates extracellular levels of the chemokine CXCL12 …

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Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2023 Jun 12, 2023

PMID: 37398044 PMCID: PMC10312657 DOI: 10.1101/2023.06.12.544689

Claudins are a family of ∼25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. Humans have 27 subtypes, which homo- and hetero-oligomerize to …

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Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Satchal K. Erramilli, Pawel K. Dominik, Dawid Deneka , Piotr Tokarz , Sangwoo S. Kim, Bharat G. Reddy, Blazej M. Skrobek , Olivier Dalmas , Eduardo Perozo, Anthony A. Kossiakoff 

J Mol Biol. 2023

PMD: 37394032 PMID: 37394032 DOI: 10.1016/j.jmb.2023.168192

CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg2+, and highly asymmetric …

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Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Karthik Srinivasan, Satchal K. Erramilli, Srinivas Chakravarthy, Adrian Gonzalez, Anthony Kossiakoff, Nicholas Noinaj

Structure. 2023 Mar 17; S0969-2126(23)00078-3.

PMID: 36977410 DOI: 10.1016/j.str.2023.03.002

Roughly 95% of the proteins that make up the chloroplast must be imported from the cytoplasm. The machinery responsible for the translocation of these cargo proteins is called the translocon at the outer …

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Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder

Szymon P Kordon, Przemysław Dutka, Justyna M Adamska, Sumit J Bandekar, Katherine Leon, Satchal K Erramilli, Brock Adams, Jingxian Li, Anthony A Kossiakoff, Demet Araç

Nat Commun. 2023 Feb 6

PMID: 36746957 PMCID: PMC9902482 DOI: 10.1038/s41467-023-36312-7

Adhesion G protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple ligands to regulate …

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A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel

Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport

Nature. 2022 Jun 29

PMID: 35768507 DOI: 10.1038/s41586-022-04903-x

Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then …

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Architecture of the cytoplasmic face of the nuclear pore

Christopher J Bley, Si Nie, George W Mobbs, Stefan Petrovic, Anna T Gres, Xiaoyu Liu, Somnath Mukherjee, Sho Harvey, Ferdinand M Huber, Daniel H Lin, Bonnie Brown, Aaron W Tang, Emily J Rundlet, Ana R Correia, Shane Chen, Saroj G Regmi, Taylor A Stevens, Claudia A Jette, Mary Dasso, Alina Patke, Alexander F Palazzo, Anthony …

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