Category: publications

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Kadidia Samassekou, Elisabeth E. Garland-Kuntz, Vaani Ohri, Isaac J. Fisher, Satchal K. Erramilli,Kaushik Muralidharan, Livia M. Bogdan, Abigail M. Gick, Anthony A. Kossiakoff, Angeline M. Lyon

bioRxiv 2024 September 12

PMID: 39314324 PMCID: PMC11418990 DOI: 10.1101/2024.09.11.612521

Phospholipase Cε (PLCε) increases intracellular Ca 2+ and protein kinase C (PKC) activity in the cardiovascular system in response …

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Structural insights into translocation and tailored synthesis of hyaluronan

Ireneusz Górniak, Zachery Stephens, Satchal K. Erramilli, Tomasz Gawda, Anthony A. Kossiakoff & Jochen Zimmer

Nature Structural & Molecular Biology (2024)

PMID: 39322765 DOI: 10.1038/s41594-024-01389-1

Hyaluronan (HA) is an essential component of the vertebrate extracellular matrix. It is a heteropolysaccharide of N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcA) reaching several megadaltons in healthy tissues. HA is …

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Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Yaqi Liu, Chelsea M Brown, Nuno Borges, Rodrigo N Nobre, Satchal Erramilli, Meagan Belcher Dufrisne, Brian Kloss, Sabrina Giacometti, Ana M Esteves, Cristina G Timoteo, Piotr Tokarz, Rosemary Cater, Yasu S Morita, Anthony A Kossiakoff, Helena Santos, Phillip J Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 18

PMID: 39345506 PMCID: PMC11429742 DOI: 10.1101/2024.09.17.613550

Tuberculosis …

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Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Yaqi Liu, Chelsea M. Brown, Satchal Erramilli, Yi-Chia Su, Po-Sen Tseng, Yu-Jen Wang, Nam Ha Duong, Piotr Tokarz, Brian Kloss, Cheng-Ruei Han, Hung-Yu Chen, José Rodrigues, Margarida Archer, Todd L. Lowary, Anthony A. Kossiakoff, Phillip J. Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 17

PMID: 39345558 PMCID: PMC11429727 DOI: 10.1101/2024.09.17.613533

The emergence of drug-resistant …

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Engineered Protein-G variants for plug-and-play applications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

bioRxiv 2024 August 08.

PMID: 39211271 PMCID: PMC11361062 DOI: 10.1101/2024.08.06.606809

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are …

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Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which contains several minor vault components (MVCs) and …

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Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

Science Direct Structure 8 October 2024

PMID: 39383874 DOI:10.1016/j.str.2024.09.015

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE binds selectively to membrane protein receptors called claudins on …

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Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial …

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Development of Luminescent Biosensors for Calprotectin

Tong Lan, Tomasz Slezak, Jinyue Pu, Julia Zinkus-Boltz, Sarbani Adhikari, Joel R. Pekow, Vibha Taneja, Joaquin Zuniga, Itzel A. Gómez-García, Nora Regino-Zamarripa, Mushtaq Ahmed, Shabaana A. Khader, David T. Rubin, Anthony A. Kossiakoff, and Bryan C. Dickinson

ACS Chem. Biol. June 6, 2024 © 2024 American Chemical Society

PMID: 38843544 DOI: 10.1021/acschembio.4c00265

Calprotectin, a metal …

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The Molecular Basis of Human ALKBH3 Mediated RNA N1-methyladenosine (m1A) Demethylation

Lin Zhang, Hong-Chao Duan, Marcin Paduch, Jingyan Hu, Chi Zhang, Yajuan Mu, Houwen Lin, Chuan He, Anthony A. Kossiakoff, Guifang Jia, Liang Zhang

Zitierweise: Angew. Chem. Int. Ed. 2024, 63, e202313900

PMID: 38158383 DOI: 10.1002/ange.202313900

N1-methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, and the distribution and dynamics of the modification play key epitranscriptomic roles …

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