Author's posts
Nov 01 2016
The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical
Galilee M, Britan-Rosich E, Griner SL, Uysal S, Baumgärtel V, Lamb DC, Kossiakoff AA, Kotler M, Stroud RM, Marx A, Alian A
Structure 2016 11;24(11):1936-1946
PMID: 27692964
Abstract
HIV-1 integrase (IN) catalyzes viral DNA integration into the host genome and facilitates multifunctional steps including virus particle maturation. Competency of IN to form multimeric assemblies is …
Oct 09 2016
Specific Recognition of a Single-Stranded RNA Sequence by a Synthetic Antibody Fragment
Shao Y, Huang H, Qin D, Li NS, Koide A, Staley JP, Koide S, Kossiakoff AA, Piccirilli JA
J. Mol. Biol. 2016 10;428(20):4100-4114
PMID: 27593161
Abstract
Antibodies that bind RNA represent an unrealized source of reagents for synthetic biology and for characterizing cellular transcriptomes. However, facile access to RNA-binding antibodies requires the engineering of effective …
Mar 09 2016
A polar ring endows improved specificity to an antibody fragment
Schaefer ZP, Bailey LJ, Kossiakoff AA
Protein Sci. 2016 07;25(7):1290-8
PMID: 27334407
Abstract
Engineering monovalent Fab fragments into bivalent formats like IgGs or F(ab’)2 can lead to aggregation presumably because of nonspecific off-target interactions that induce aggregation. In an effort to further understand the molecular determinants of nonspecific interactions for engineered antibodies and natively folded …
Feb 02 2016
Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs
Dominik PK, Borowska MT, Dalmas O, Kim SS, Perozo E, Keenan RJ, Kossiakoff AA
Structure 2016 Feb;24(2):300-9
PMID: 26749445
Abstract
A major challenge in membrane biophysics is to define the mechanistic linkages between a protein’s conformational transitions and its function. We describe a novel approach to stabilize transient functional states of membrane proteins in native-like …
Oct 05 2015
Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins
Zhong N, Loppnau P, Seitova A, Ravichandran M, Fenner M, Jain H, Bhattacharya A, Hutchinson A, Paduch M, Lu V, Olszewski M, Kossiakoff AA, Dowdell E, Koide A, Koide S, Huang H, Nadeem V, Sidhu SS, Greenblatt JF, Marcon E, Arrowsmith CH, Edwards AM, Gräslund S
PLoS ONE 2015;10(10):e0139695
PMID: 26437229
Abstract
We developed and …
Oct 02 2015
Architecture of the fungal nuclear pore inner ring complex
Stuwe T, Bley CJ, Thierbach K, Petrovic S, Schilbach S, Mayo DJ, Perriches T, Rundlet EJ, Jeon YE, Collins LN, Huber FM, Lin DH, Paduch M, Koide A, Lu V, Fischer J, Hurt E, Koide S, Kossiakoff AA, Hoelz A
Science 2015 Oct;350(6256):56-64
PMID: 26316600
Abstract
The nuclear pore complex (NPC) constitutes the sole gateway …
Aug 19 2015
A High Through-put Platform for Recombinant Antibodies to Folded Proteins
Hornsby M, Paduch M, Miersch S, Sääf A, Matsuguchi T, Lee B, Wypisniak K, Doak A, King D, Usatyuk S, Perry K, Lu V, Thomas W, Luke J, Goodman J, Hoey RJ, Lai D, Griffin C, Li Z, Vizeacoumar FJ, Dong D, Campbell E, Anderson S, Zhong N, Gräslund S, Koide S, Moffat J, Sidhu …
May 06 2015
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation
Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong …
Mar 06 2015
Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo
Mateja A, Paduch M, Chang HY, Szydlowska A, Kossiakoff AA, Hegde RS, Keenan RJ
Science 2015 Mar;347(6226):1152-5
PMID: 25745174
Abstract
Tail-anchored (TA) proteins are a physiologically important class of membrane proteins targeted to the endoplasmic reticulum by the conserved guided-entry of TA proteins (GET) pathway. During transit, their hydrophobic transmembrane domains (TMDs) are chaperoned by …
Mar 06 2015
Nuclear pores. Architecture of the nuclear pore complex coat
Stuwe T, Correia AR, Lin DH, Paduch M, Lu VT, Kossiakoff AA, Hoelz A
Science 2015 Mar;347(6226):1148-52
PMID: 25745173
Abstract
The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a …