Author's posts

Generating conformation-specific synthetic antibodies to trap proteins in selected functional states

Paduch M, Koide A, Uysal S, Rizk SS, Koide S, Kossiakoff AA

Methods 2013 Mar;60(1):3-14

PMID: 23280336


A set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties. Exquisite control of antigen and conditions during the phage display selection process …

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Structural Modeling of Human Growth Hormone Receptor using Computational Simulations and NMR Spectroscopy

Rui H, Kossiakoff AA, Im W

Biophysical Journal, Volume 104, Issue 2, pp. 117a-118a


Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …

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Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo

Rizk SS, Misiura A, Paduch M, Kossiakoff AA

Bioconjug. Chem. 2012 Jan;23(1):42-6

PMID: 22175275


The use of proteins or nucleic acids as therapeutic agents has been severely hampered by their intrinsic inability to cross the cell membrane. Moreover, common techniques for driving the delivery of macromolecules lack the ability to distinguish between healthy and …

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