Author's posts
Jan 17 2015
Scalable high throughput selection from phage-displayed synthetic antibody libraries
Miersch S, Li Z, Hanna R, McLaughlin ME, Hornsby M, Matsuguchi T, Paduch M, Sääf A, Wells J, Koide S, Kossiakoff A, Sidhu SS
J Vis Exp 2015 Jan;(95):51492
PMID: 25651360
Abstract
The demand for antibodies that fulfill the needs of both basic and clinical research applications is high and will dramatically increase in the …
Jan 15 2015
Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling
Rizk SS, Kouadio JL, Szymborska A, Duguid EM, Mukherjee S, Zheng J, Clevenger CV, Kossiakoff AA
Cell Commun. Signal 2015 Jan;13:1
PMID: 25589173
Abstract
BACKGROUND: Many receptors function by binding to multiple ligands, each eliciting a distinct biological output. The extracellular domain of the human prolactin receptor (hPRL-R) uses an identical epitope to bind to …
Dec 15 2014
Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments
Bailey LJ, Sheehy KM, Hoey RJ, Schaefer ZP, Ura M, Kossiakoff AA
J. Immunol. Methods 2014 Dec;415:24-30
PMID: 25450256
Abstract
Immunoglobulin binding proteins (IBPs) are broadly used as reagents for the purification and detection of antibodies. Among the IBPs, the most widely used are Protein-A and Protein-G. The C2 domain of Protein-G from Streptococcus is …
Dec 12 2014
A synthetic antibody fragment targeting nicastrin affects assembly and trafficking of γ-secretase
Zhang X, Hoey R, Koide A, Dolios G, Paduch M, Nguyen P, Wu X, Li Y, Wagner SL, Wang R, Koide S, Sisodia SS
J. Biol. Chem. 2014 Dec;289(50):34851-61
PMID: 25352592
Abstract
The γ-secretase complex, composed of presenilin, nicastrin (NCT), anterior pharynx-defective 1 (APH-1), and presenilin enhancer 2 (PEN-2), is assembled in a highly regulated …
Aug 14 2014
Visualization of arrestin recruitment by a G-protein-coupled receptor
Shukla AK, Westfield GH, Xiao K, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu SS, Koide S, Penczek PA, Kossiakoff AA, Woods VL, Kobilka BK, Skiniotis …
Aug 13 2014
Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies
Welch BD, Paduch M, Leser GP, Bergman Z, Kors CA, Paterson RG, Jardetzky TS, Kossiakoff AA, Lamb RA
J. Virol. 2014 Oct;88(20):11713-25
PMID: 25122782
Abstract
Paramyxoviruses are enveloped negative-strand RNA viruses that are significant human and animal pathogens. Most paramyxoviruses infect host cells via the concerted action of a tetrameric attachment protein (variously called HN, …
Jul 10 2014
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation
Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J
Cell Rep 2014 Jul;8(1):297-310
PMID: …
Feb 12 2014
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain
Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E
Nat. Struct. Mol. Biol. 2014 Mar;21(3):244-52
PMID: 24487958
Abstract
The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing …
May 16 2013
New Tricks for an Old Dimer
Wells JA, Kossiakoff AA
Cell Biology, Volume 344, Issue 6185, pp. 703-704
Abstract
Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …
Apr 21 2013
Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide
Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ
Nature 2013 May;497(7447):137-41
PMID: 23604254
Abstract
The functions of G-protein-coupled receptors (GPCRs) are primarily mediated and modulated by three …