Author's posts

Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments

Bailey LJ, Sheehy KM, Hoey RJ, Schaefer ZP, Ura M, Kossiakoff AA

J. Immunol. Methods 2014 Dec;415:24-30

PMID: 25450256

Abstract

Immunoglobulin binding proteins (IBPs) are broadly used as reagents for the purification and detection of antibodies. Among the IBPs, the most widely used are Protein-A and Protein-G. The C2 domain of Protein-G from Streptococcus is …

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A synthetic antibody fragment targeting nicastrin affects assembly and trafficking of γ-secretase

Zhang X, Hoey R, Koide A, Dolios G, Paduch M, Nguyen P, Wu X, Li Y, Wagner SL, Wang R, Koide S, Sisodia SS

J. Biol. Chem. 2014 Dec;289(50):34851-61

PMID: 25352592

Abstract

The γ-secretase complex, composed of presenilin, nicastrin (NCT), anterior pharynx-defective 1 (APH-1), and presenilin enhancer 2 (PEN-2), is assembled in a highly regulated …

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Visualization of arrestin recruitment by a G-protein-coupled receptor

Shukla AK, Westfield GH, Xiao K, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu SS, Koide S, Penczek PA, Kossiakoff AA, Woods VL, Kobilka BK, Skiniotis …

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Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies

Welch BD, Paduch M, Leser GP, Bergman Z, Kors CA, Paterson RG, Jardetzky TS, Kossiakoff AA, Lamb RA

J. Virol. 2014 Oct;88(20):11713-25

PMID: 25122782

Abstract

Paramyxoviruses are enveloped negative-strand RNA viruses that are significant human and animal pathogens. Most paramyxoviruses infect host cells via the concerted action of a tetrameric attachment protein (variously called HN, …

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Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Cell Rep 2014 Jul;8(1):297-310

PMID: …

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Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain

Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E

Nat. Struct. Mol. Biol. 2014 Mar;21(3):244-52

PMID: 24487958

Abstract

The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing …

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New Tricks for an Old Dimer

Wells JA, Kossiakoff AA

Cell Biology, Volume 344, Issue 6185, pp. 703-704

Abstract

Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …

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Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide

Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ

Nature 2013 May;497(7447):137-41

PMID: 23604254

Abstract

The functions of G-protein-coupled receptors (GPCRs) are primarily mediated and modulated by three …

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Generating conformation-specific synthetic antibodies to trap proteins in selected functional states

Paduch M, Koide A, Uysal S, Rizk SS, Koide S, Kossiakoff AA

Methods 2013 Mar;60(1):3-14

PMID: 23280336

Abstract

A set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties. Exquisite control of antigen and conditions during the phage display selection process …

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Structural Modeling of Human Growth Hormone Receptor using Computational Simulations and NMR Spectroscopy

Rui H, Kossiakoff AA, Im W

Biophysical Journal, Volume 104, Issue 2, pp. 117a-118a

Abstract

Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …

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