Jan 18 2012
Rizk SS, Misiura A, Paduch M, Kossiakoff AA
Bioconjug. Chem. 2012 Jan;23(1):42-6
PMID: 22175275
Abstract
The use of proteins or nucleic acids as therapeutic agents has been severely hampered by their intrinsic inability to cross the cell membrane. Moreover, common techniques for driving the delivery of macromolecules lack the ability to distinguish between healthy and …
Jul 08 2011
Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E
Proc. Natl. Acad. Sci. U.S.A. 2011 Jul;108(29):11896-9
PMID: 21730186
Abstract
Using a constitutively active channel mutant, we solved the structure of full-length KcsA in the open conformation at 3.9 Å. The structure reveals that the activation gate expands about 20 Å, exerting a strain on …
Apr 08 2011
Rizk SS, Paduch M, Heithaus JH, Duguid EM, Sandstrom A, Kossiakoff AA
Nat. Struct. Mol. Biol. 2011 Apr;18(4):437-42
PMID: 21378967
Abstract
We describe a phage display methodology for engineering synthetic antigen binders (sABs) that recognize either the apo or the ligand-bound conformation of maltose-binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP …
Jan 08 2011
Koldobskaya Y, Duguid EM, Shechner DM, Suslov NB, Ye J, Sidhu SS, Bartel DP, Koide S, Kossiakoff AA, Piccirilli JA
Nat. Struct. Mol. Biol. 2011 Jan;18(1):100-6
PMID: 21151117
Abstract
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic …
Nov 08 2010
Brawley CM, Uysal S, Kossiakoff AA, Rock RS
PLoS ONE 2010 Nov;5(11):e13960
PMID: 21103060
Abstract
BACKGROUND: Eukaryotic cells strictly regulate the structure and assembly of their actin filament networks in response to various stimuli. The actin binding proteins that control filament assembly are therefore attractive targets for those who wish to reorganize actin filaments and …
Mar 08 2010
Kossiakoff AA, Rizk S
Structure 2010 Mar;18(3):275-6
PMID: 20223207
Abstract
Cytokine signaling is triggered by a hormone-induced receptor aggregation process. IL-13 employs an unconventional sequence of steps for assembling its signaling complex to trigger activation and for turning it off. Both these processes involve some unusual molecular recognition features, as discussed by Lupardus et al. …
Jul 08 2009
Rizk SS, Luchniak A, Uysal S, Brawley CM, Rock RS, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Jul;106(27):11011-5
PMID: 19549879
Abstract
We have developed and tested a robust delivery method for the transport of proteins to the cytoplasm of mammalian cells without compromising the integrity of the cell membrane. This receptor-mediated delivery (RMD) technology …
Jul 08 2009
Bang D, Tereshko V, Kossiakoff AA, Kent SB
Mol Biosyst 2009 Jul;5(7):750-6
PMID: 19562114
Abstract
We have used total chemical synthesis to prepare [V15A]crambin-alpha-carboxamide, a unique protein analogue that eliminates a salt bridge between the delta-guanidinium of the Arg(10) side chain and the alpha-carboxylate of Asn(46) at the C-terminus of the polypeptide chain. This salt …
Jun 08 2009
Mandal K, Pentelute BL, Tereshko V, Thammavongsa V, Schneewind O, Kossiakoff AA, Kent SB
Protein Sci. 2009 Jun;18(6):1146-54
PMID: 19472324
Abstract
We describe the use of racemic crystallography to determine the X-ray structure of the natural product plectasin, a potent antimicrobial protein recently isolated from fungus. The protein enantiomers L-plectasin and D-plectasin were prepared by …
Apr 08 2009
Uysal S, Vásquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A
Proc. Natl. Acad. Sci. U.S.A. 2009 Apr;106(16):6644-9
PMID: 19346472
Abstract
KcsA is a proton-activated, voltage-modulated K(+) channel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments …