Aug 13 2014
Welch BD, Paduch M, Leser GP, Bergman Z, Kors CA, Paterson RG, Jardetzky TS, Kossiakoff AA, Lamb RA
J. Virol. 2014 Oct;88(20):11713-25
PMID: 25122782
Abstract
Paramyxoviruses are enveloped negative-strand RNA viruses that are significant human and animal pathogens. Most paramyxoviruses infect host cells via the concerted action of a tetrameric attachment protein (variously called HN, …
Jul 10 2014
Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J
Cell Rep 2014 Jul;8(1):297-310
PMID: …
Feb 12 2014
Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E
Nat. Struct. Mol. Biol. 2014 Mar;21(3):244-52
PMID: 24487958
Abstract
The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing …
May 16 2013
Wells JA, Kossiakoff AA
Cell Biology, Volume 344, Issue 6185, pp. 703-704
Abstract
Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …
Apr 21 2013
Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ
Nature 2013 May;497(7447):137-41
PMID: 23604254
Abstract
The functions of G-protein-coupled receptors (GPCRs) are primarily mediated and modulated by three …
Mar 15 2013
Paduch M, Koide A, Uysal S, Rizk SS, Koide S, Kossiakoff AA
Methods 2013 Mar;60(1):3-14
PMID: 23280336
Abstract
A set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties. Exquisite control of antigen and conditions during the phage display selection process …
Jan 18 2012
Rizk SS, Misiura A, Paduch M, Kossiakoff AA
Bioconjug. Chem. 2012 Jan;23(1):42-6
PMID: 22175275
Abstract
The use of proteins or nucleic acids as therapeutic agents has been severely hampered by their intrinsic inability to cross the cell membrane. Moreover, common techniques for driving the delivery of macromolecules lack the ability to distinguish between healthy and …
Jul 08 2011
Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E
Proc. Natl. Acad. Sci. U.S.A. 2011 Jul;108(29):11896-9
PMID: 21730186
Abstract
Using a constitutively active channel mutant, we solved the structure of full-length KcsA in the open conformation at 3.9 Å. The structure reveals that the activation gate expands about 20 Å, exerting a strain on …
Apr 08 2011
Rizk SS, Paduch M, Heithaus JH, Duguid EM, Sandstrom A, Kossiakoff AA
Nat. Struct. Mol. Biol. 2011 Apr;18(4):437-42
PMID: 21378967
Abstract
We describe a phage display methodology for engineering synthetic antigen binders (sABs) that recognize either the apo or the ligand-bound conformation of maltose-binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP …
Jan 08 2011
Koldobskaya Y, Duguid EM, Shechner DM, Suslov NB, Ye J, Sidhu SS, Bartel DP, Koide S, Kossiakoff AA, Piccirilli JA
Nat. Struct. Mol. Biol. 2011 Jan;18(1):100-6
PMID: 21151117
Abstract
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic …