Judy Luke
Author's posts
Nov 30 2019
Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM
Dutka P, Mukherjee S, Gao X, Kang Y, de Waal PW, Wang L, Zhuang Y, Melcher K, Zhang C, Xu HE, Kossiakoff AA
Structure 2019 Oct;
PMID: 31669042
Abstract
“Universal” synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling …
Oct 18 2019
An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability
Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA
Protein Sci. 2019 Oct;
PMID: 31622515
Abstract
Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to …
Jul 07 2019
Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin
Farcasanu M, Wang AG, Uchański T, Bailey LJ, Yue J, Chen Z, Wu X, Kossiakoff A, Tang WJ
Biochemistry 2019 Jun;
PMID: 31243996
Abstract
Anthrax, a lethal, weaponizable disease caused by Bacillus anthracis, acts through exotoxins that are primary mediators of systemic toxicity and also targets for neutralization by passive immunotherapy. The ease of engineering …
Sep 17 2018
Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2
Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S
Proc. Natl. Acad. Sci. U.S.A. 2018 Sep;
PMID: 30224453
Abstract
The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational modifications associated with …
Sep 06 2018
Structural basis for activation of voltage sensor domains in an ion channel TPC1
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
Proc. Natl. Acad. Sci. U.S.A. 2018 Sep;
PMID: 30190435
Abstract
Voltage-sensing domains (VSDs) couple changes in transmembrane electrical potential to conformational changes that regulate ion conductance through a central channel. Positively charged amino …
Jun 27 2018
The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab
Radwańska MJ, Jaskółowski M, Davydova E, Derewenda U, Miyake T, Engel DA, Kossiakoff AA, Derewenda ZS
Acta Crystallogr D Struct Biol 2018 Jul;74(Pt 7):681-689
PMID: 29968677
Abstract
The vast majority of platforms for the detection of viral or bacterial antigens rely on immunoassays, typically ELISA or sandwich ELISA, that are contingent on the availability of …
Jun 18 2018
Cryo-EM structure of human rhodopsin bound to an inhibitory G protein
Kang Y, Kuybeda O, de Waal PW, Mukherjee S, Van Eps N, Dutka P, Zhou XE, Bartesaghi A, Erramilli S, Morizumi T, Gu X, Yin Y, Liu P, Jiang Y, Meng X, Zhao G, Melcher K, Ernst OP, Kossiakoff AA, Subramaniam S, Xu HE
Nature 2018 Jun;
PMID: 29899450
Abstract
G-protein-coupled receptors comprise the largest …
Apr 27 2018
Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
Elife 2018 Mar;7
PMID: 29596046
Abstract
Insulin degrading enzyme (IDE) plays key roles …
Apr 27 2018
Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination
Bailey LJ, Sheehy KM, Dominik PK, Liang WG, Rui H, Clark M, Jaskolowski M, Kim Y, Deneka D, Tang WJ, Kossiakoff AA
J. Mol. Biol. 2018 Feb;430(3):337-347
PMID: 29273204
Abstract
Antibody Fab fragments have been exploited with significant success to facilitate the structure determination of challenging macromolecules as crystallization chaperones and as molecular fiducial marks …
Aug 15 2015
A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction
Mukherjee S, Ura M, Hoey RJ, Kossiakoff AA
J. Mol. Biol. 2015 Aug;427(16):2707-25
PMID: 26159704
Abstract
Reversible, high-affinity immobilization tags are critical tools for myriad biological applications. However, inherent issues are associated with a number of the current methods of immobilization. Particularly, a critical element in phage display sorting is functional immobilization of target proteins. …