Judy Luke
Author's posts
Nov 08 2001
High affinity RNase S-peptide variants obtained by phage display have a novel “hot-spot” of binding energy
Dwyer JJ, Dwyer MA, Kossiakoff AA
Biochemistry 2001 Nov;40(45):13491-500
PMID: 11695896
Abstract
Using phage display mutagenesis, high affinity variants of RNase S-peptide were produced that bind to RNase S-protein over 100-fold more tightly than the wild type S-peptide. The S-peptide: S-protein interface was further characterized using “biased” phage display libraries, where each targeted residue was …
Apr 08 2000
Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity
Dwyer MA, Lu W, Dwyer JJ, Kossiakoff AA
Chem. Biol. 2000 Apr;7(4):263-74
PMID: 10780926
Abstract
BACKGROUND: Molecular diversity in nature is developed through a combination of genetic and chemical elements. We have developed a method that permits selective manipulation of both these elements in one protein engineering tool. It combines the ability to introduce non-natural …