Judy Luke

Author's posts

Synthetic antibody binders detect distinct cellular states of Chromosome Passenger Complex proteins

Marcin Ura, Somnath Mukherjee, Edyta Marcon, Stefan A Koestler, Anthony A Kossiakoff

J Mol Biol. 2022 Apr 22;167602.

PMID: 35469831 DOI: 10.1016/j.jmb.2022.167602

High-performance affinity reagents are essential tools to enable biologists to profile the cellular location and composition of macromolecular complexes undergoing dynamic reorganization. To support further development of such tools, we have assembled a …

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Structural basis of lipopolysaccharide maturation by the O-antigen ligase

Khuram U. Ashraf, Rie Nygaard, Owen N. Vickery, Satchal K. Erramilli, Carmen M. Herrera, Thomas H. McConville, Vasileios I. Petrou, Sabrina I. Giacometti, Meagan Belcher Dufrisne, Kamil Nosol, Allen P. Zinkle, Chris L. B. Graham, Michael Loukeris, Brian Kloss, Karolina Skorupinska-Tudek, Ewa Swiezewska, David I. Roper, Oliver B. Clarke, Anne-Catrin Uhlemann, Anthony A. Kossiakoff, M. …

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Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers

Shion A Lim, Jie Zhou, Alexander J Martinko, Yung-Hua Wang, Ekaterina V Filippova, Veronica Steri, Donghui Wang, Soumya G Remesh, Jia Liu, Byron Hann, Anthony A Kossiakoff, Michael J Evans, Kevin K Leung, James A Wells

J Clin Invest. 2022 Feb 15;132(4):e154604.

PMID: 35166238 PMCID: PMC8843743 DOI: 10.1172/JCI154604

Extracellular proteolysis is frequently dysregulated in disease and can generate …

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Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels

Ahmed Rohaim, Tomasz Slezak, Young Hoon Koh, Lydia Blachowicz, Anthony A Kossiakoff, Benoît Roux

J Gen Physiol. 2022 Apr 4;154(4):e202112965.

PMID: 35234830 DOI: 10.1085/jgp.202112965

Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid structural determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges of routine …

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)

PMID: 34782475 DOI: 10.1073/pnas.2115435118

With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …

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Structures of ABCB4 provide insight into phosphatidylcholine translocation

Kamil Nosol, Rose Bang-Sørensen, Rossitza N Irobalieva, Satchal K Erramilli, Bruno Stieger, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci USA 2021 Aug 17;118(33):e2106702118. 

PMID: 34385322 PMCID: PMC8379956 (available on 2022-02-12)

DOI: 10.1073/pnas.2106702118

ABCB4 is expressed in hepatocytes and translocates phosphatidylcholine into bile canaliculi. The mechanism of specific lipid recruitment from the canalicular …

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Structure of an AMPK complex in an inactive, ATP-bound state

Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher

Science 23 Jul 2021: …

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Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1

Qiuyan Chen, Manolo Plasencia, Zhuang Li, Somnath Mukherjee, Dhabaleswar Patra, Chun-Liang Chen, Thomas Klose, Xin-Qiu Yao, Anthony A Kossiakoff, Leifu Chang, Philip C Andrews, John J G Tesmer

Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x

PMID: 34262173

G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization1. Although it is unclear how …

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K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle

Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff, Lei Liu & Minglei Zhao

Nat Chem Biol. 2021 Jul 8. doi: 10.1038/s41589-021-00823-5

PMID: 34239127

Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin via different linkages. Deciphering the cellular ubiquitin …

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A T cell redirection platform for co-targeting dual antigens on solid tumors

Leonie Enderle, Karim H Shalaby, Maryna Gorelik, Alexander Weiss, Levi L Blazer, Marcin Paduch, Lia Cardarelli, Anthony Kossiakoff, Jarrett J Adams, Sachdev S Sidhu

MAbs. Jan-Dec 2021;13(1):1933690. doi: 10.1080/19420862.2021.1933690

PMID: 34190031

In order to direct T cells to specific features of solid cancer cells, we engineered a bispecific antibody format, named Dual Antigen T cell …

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