Judy Luke
Author's posts
Mar 29 2020
Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins
Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA
Nat Commun 2020 Mar;11(1):1598
PMID: 32221310
Abstract
We propose the concept of universal fiducials based on a set of pre-made …
Feb 26 2020
Structure and mechanism of the ER-based glucosyltransferase ALG6
Bloch JS, Pesciullesi G, Boilevin J, Nosol K, Irobalieva RN, Darbre T, Aebi M, Kossiakoff AA, Reymond JL, Locher KP
Nature 2020 03;579(7799):443-447
PMID: 32103179
Abstract
In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins. The final seven steps occur in the lumen …
Jan 02 2020
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science.
The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries …
Dec 11 2019
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.
Dec 02 2019
Structure and drug resistance of the Plasmodium falciparum transporter PfCRT
Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F
Nature 2019 Nov;
PMID: 31776516
Abstract
The emergence and spread of drug-resistant Plasmodium falciparum impedes global efforts to control …
Nov 30 2019
Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM
Dutka P, Mukherjee S, Gao X, Kang Y, de Waal PW, Wang L, Zhuang Y, Melcher K, Zhang C, Xu HE, Kossiakoff AA
Structure 2019 Oct;
PMID: 31669042
Abstract
“Universal” synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling …
Oct 18 2019
An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability
Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA
Protein Sci. 2019 Oct;
PMID: 31622515
Abstract
Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to …
Jul 07 2019
Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin
Farcasanu M, Wang AG, Uchański T, Bailey LJ, Yue J, Chen Z, Wu X, Kossiakoff A, Tang WJ
Biochemistry 2019 Jun;
PMID: 31243996
Abstract
Anthrax, a lethal, weaponizable disease caused by Bacillus anthracis, acts through exotoxins that are primary mediators of systemic toxicity and also targets for neutralization by passive immunotherapy. The ease of engineering …
Sep 17 2018
Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2
Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S
Proc. Natl. Acad. Sci. U.S.A. 2018 Sep;
PMID: 30224453
Abstract
The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational modifications associated with …
Sep 06 2018
Structural basis for activation of voltage sensor domains in an ion channel TPC1
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
Proc. Natl. Acad. Sci. U.S.A. 2018 Sep;
PMID: 30190435
Abstract
Voltage-sensing domains (VSDs) couple changes in transmembrane electrical potential to conformational changes that regulate ion conductance through a central channel. Positively charged amino …