Judy Luke

Author's posts

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Nature Communications volume 15, Article number: 2476 (2024)

Hongtao Liu, Dariusz Zakrzewicz, Kamil Nosol, Rossitza N Irobalieva, Somnath Mukherjee, Rose Bang-Sørensen, Nora Goldmann, Sebastian Kunz, Lorenzo Rossi, Anthony A Kossiakoff, Stephan Urban, Dieter Glebe, Joachim Geyer, Kaspar P Locher

PMID: 38509088 PMCID: PMC10954734 DOI: 10.1038/s41467-024-46706-w

Cellular entry of the hepatitis B and D viruses (HBV/HDV) …

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Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Szymon P. Kordon, Kristina Cechova, Sumit J. Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A. Kossiakoff, Reza Vafabakhsh, and Demet Araç

bioRxiv Preprint. 2024 Feb 26

PMID: 38464178 DOI: 10.1101/2024.02.25.581807

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain extracellular regions (ECR) containing a conserved …

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Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

Heather A Bruce, Alexander U Singer, Ekaterina V Filippova, Levi L Blazer, Jarrett J Adams, Leonie Enderle, Moshe Ben-David, Elizabeth H Radley, Daniel Y L Mao, Victor Pau, Stephen Orlicky, Frank Sicheri, Igor Kourinov, Shane Atwell, Anthony A Kossiakoff, Sachdev S Sidhu

Protein Sci. 2023 Nov 9:e4824.

PMID: 37945533 DOI: 10.1002/pro.4824

The atomic-resolution structural information …

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Structural and molecular basis of choline uptake into the brain by FLVCR2

Rosemary J. Cater, Dibyanti Mukherjee, Eva Gil Iturbe, Satchal K. Erramilli, Ting Chen, Katie Koo, Nicolás Santander Grez, Andrew Reckers, Brian Kloss, Tomasz Gawda, Brendon C. Choy, Zhening Zheng, Oliver B. Clarke, Sook Wah Yee, Anthony A. Kossiakoff, Matthias Quick, Thomas Arnold, Filippo Mancia

bioRxiv. 2023 Oct 5:2023.10.05.561059.

PMID: 37873173 PMCID: PMC10592973 DOI: 10.1101/2023.10.05.561059

Choline is an essential …

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Structure of human drug transporters OATP1B1 and OATP1B3

Anca-Denise Ciută, Kamil Nosol, Julia Kowal, Somnath Mukherjee, Ana S. Ramírez, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher

Nature Communications volume 14, Article number: 5774 (2023)

PMID: 37723174 PMCID: PMC10507018 DOI: 10.1038/s41467-023-41552-8

The organic anion transporting polypeptides OATP1B1 and OATP1B3 are membrane proteins that mediate uptake of drugs into the liver for subsequent conjugation and biliary …

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Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake

Paweł P Knejski, Satchal K Erramilli, Anthony A Kossiakoff

bioRxiv. 2023 Aug 1:2023.08.01.551527.

PMID: 37577460 PMCID: PMC10418163 DOI: 10.1101/2023.08.01.551527

Pathogenic bacteria, such as Pseudomonas aeruginosa , depend on scavenging heme for the acquisition of iron, an essential nutrient. The TonB-dependent transporter (TBDT) PhuR is the major heme uptake protein in P. aeruginosa clinical isolates. However, a comprehensive understanding …

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Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

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ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Qiuyan Chen, Christopher T Schafer, Somnath Mukherjee, Martin Gustavsson, Parth Agrawal, Xin-Qiu Yao, Anthony A Kossiakoff, Tracy M Handel, John J G Tesmer

bioRxiv 2023 Jul 19, 2023

PMID: 37502840 PMCID: PMC10370059 DOI: 10.1101/2023.07.18.549504

Atypical chemokine receptor 3 (ACKR3, also known as CXCR7) is a scavenger receptor that regulates extracellular levels of the chemokine CXCL12 …

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Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2023 Jun 12, 2023

PMID: 37398044 PMCID: PMC10312657 DOI: 10.1101/2023.06.12.544689

Claudins are a family of ∼25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. Humans have 27 subtypes, which homo- and hetero-oligomerize to …

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Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Satchal K. Erramilli, Pawel K. Dominik, Dawid Deneka , Piotr Tokarz , Sangwoo S. Kim, Bharat G. Reddy, Blazej M. Skrobek , Olivier Dalmas , Eduardo Perozo, Anthony A. Kossiakoff 

J Mol Biol. 2023

PMD: 37394032 PMID: 37394032 DOI: 10.1016/j.jmb.2023.168192

CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg2+, and highly asymmetric …

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