Judy Luke
Author's posts
Aug 15 2022
Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes
Benjamin J. Orlando, Pawel K. Dominik, Sourav Roy, Chinemerem P. Ogbu, Satchal K. Erramilli, AnthonyA. Kossiakoff, Alex J. Vecchio
JBC Journal of Biological Chemistry 9 August 2022, 102357
DOI: jbc.2022.102357
Strains of Clostridium perfringens produce a two-domain enterotoxin (CpE) that afflicts humans and domesticated animals, causing prevalent gastrointestinal illnesses. CpE’s C-terminal domain (cCpE) binds cell …
Aug 08 2022
Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias
Yu-Chen Yen, Christopher T Schafer, Martin Gustavsson, Stefanie A Eberle, Pawel K Dominik, Dawid Deneka, Penglie Zhang, Thomas J Schall, Anthony A Kossiakoff, John J G Tesmer, Tracy M Handel
Sci Adv. 2022 Jul 15;8(28):eabn8063.
PMID: 35857509 PMCID: PMC9278869 DOI: 10.1126/sciadv.abn8063
Both CXC chemokine receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) are activated by …
Jul 05 2022
A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel
Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport
Nature. 2022 Jun 29
PMID: 35768507 DOI: 10.1038/s41586-022-04903-x
Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4. Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then …
Jul 05 2022
Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver
Hongtao Liu, Rossitza N. Irobalieva, Rose Bang-Sørensen, Kamil Nosol, Somnath Mukherjee, Parth Agrawal, Bruno Stieger, Anthony A. Kossiakoff & Kaspar P. Locher
Cell Research (2022) 0:1–4
PMID: 35726088 DOI: 10.1038/s41422-022-00680-4
The sodium taurocholate (TC) co-transporting polypeptide NTCP (SLC10A1) is a secondary active membrane transport protein that mediates the uptake of bile salts from the portal blood plasma …
Jul 05 2022
Architecture of the cytoplasmic face of the nuclear pore
Christopher J Bley, Si Nie, George W Mobbs, Stefan Petrovic, Anna T Gres, Xiaoyu Liu, Somnath Mukherjee, Sho Harvey, Ferdinand M Huber, Daniel H Lin, Bonnie Brown, Aaron W Tang, Emily J Rundlet, Ana R Correia, Shane Chen, Saroj G Regmi, Taylor A Stevens, Claudia A Jette, Mary Dasso, Alina Patke, Alexander F Palazzo, Anthony …
Jul 05 2022
Quaternary structure independent folding of voltage-gated ion channel pore domain subunits
Cristina Arrigoni, Marco Lolicato, David Shaya, Ahmed Rohaim, Felix Findeisen, Lam-Kiu Fong, Claire M Colleran, Pawel Dominik, Sangwoo S Kim, Jonathan P Schuermann, William F DeGrado, Michael Grabe, Anthony A Kossiakoff, Daniel L Minor
Nat Struct Mol Biol. 2022 Jun;29(6):537-548.
PMID: 35655098 DOI: 10.1038/s41594-022-00775-x
Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from …
May 13 2022
Synthetic antibody binders detect distinct cellular states of Chromosome Passenger Complex proteins
Marcin Ura, Somnath Mukherjee, Edyta Marcon, Stefan A Koestler, Anthony A Kossiakoff
J Mol Biol. 2022 Apr 22;167602.
PMID: 35469831 DOI: 10.1016/j.jmb.2022.167602
High-performance affinity reagents are essential tools to enable biologists to profile the cellular location and composition of macromolecular complexes undergoing dynamic reorganization. To support further development of such tools, we have assembled a …
Apr 07 2022
Structural basis of lipopolysaccharide maturation by the O-antigen ligase
Khuram U. Ashraf, Rie Nygaard, Owen N. Vickery, Satchal K. Erramilli, Carmen M. Herrera, Thomas H. McConville, Vasileios I. Petrou, Sabrina I. Giacometti, Meagan Belcher Dufrisne, Kamil Nosol, Allen P. Zinkle, Chris L. B. Graham, Michael Loukeris, Brian Kloss, Karolina Skorupinska-Tudek, Ewa Swiezewska, David I. Roper, Oliver B. Clarke, Anne-Catrin Uhlemann, Anthony A. Kossiakoff, M. …
Mar 07 2022
Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers
Shion A Lim, Jie Zhou, Alexander J Martinko, Yung-Hua Wang, Ekaterina V Filippova, Veronica Steri, Donghui Wang, Soumya G Remesh, Jia Liu, Byron Hann, Anthony A Kossiakoff, Michael J Evans, Kevin K Leung, James A Wells
J Clin Invest. 2022 Feb 15;132(4):e154604.
PMID: 35166238 PMCID: PMC8843743 DOI: 10.1172/JCI154604
Extracellular proteolysis is frequently dysregulated in disease and can generate …
Mar 04 2022
Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels
Ahmed Rohaim, Tomasz Slezak, Young Hoon Koh, Lydia Blachowicz, Anthony A Kossiakoff, Benoît Roux
J Gen Physiol. 2022 Apr 4;154(4):e202112965.
PMID: 35234830 DOI: 10.1085/jgp.202112965
Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid structural determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges of routine …