Tag: cryogenic electron microscopy

Molecular characterization of the archaic HLA-B*73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2

Philipp Ross, Hugo G. Hilton, Jane Lodwick, Tomasz Slezak, Lisbeth A. Guethlein, Curtis P. McMurtrey, Alex S. Han, Morten Nielsen, Daniel Yong, Charles L. Dulberger, Kristof T. Nolan, Sobhan Roy, Caitlin D. Castro, William H. Hildebrand, Minglei Zhao, Anthony Kossiakoff, Peter Parham, Erin J. Adams

bioRxiv 2024 Nov 28:2024.11.25.625330.

PMID: 39651149 PMCID: PMC11623575 DOI: 10.1101/2024.11.25.625330

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Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function

Szymon P Kordon, Kristina Cechova, Sumit J Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A Kossiakoff, Reza Vafabakhsh, Demet Araç

Nature Communications 2024 Dec 4;15:10545.

PMID: 39627215 PMCID: PMC11615224 DOI: 10.1038/s41467-024-54836-4

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain extracellular regions (ECRs) containing a …

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Biophysical Basis of Paracellular Barrier Modulation by a Pan-Claudin-Binding Molecule

Chinemerem P Ogbu, Alexandria M Mandriota, Xiangdong Liu, Mason de las Alas, Srajan Kapoor, Jagrity Choudhury, Anthony A Kossiakoff, Michael E Duffey, Alex J Vecchio

bioRxiv 2024 Nov 11:2024.11.10

PMID: 39605593 PMCID: PMC11601404 DOI: 10.1101/2024.11.10.622873

Claudins are a 27-member protein family that form and fortify specialized cell contacts in endothelium and epithelium called tight junctions. …

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Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Kadidia Samassekou, Elisabeth E. Garland-Kuntz, Vaani Ohri, Isaac J. Fisher, Satchal K. Erramilli,Kaushik Muralidharan, Livia M. Bogdan, Abigail M. Gick, Anthony A. Kossiakoff, Angeline M. Lyon

bioRxiv 2024 September 12

PMID: 39314324 PMCID: PMC11418990 DOI: 10.1101/2024.09.11.612521

Phospholipase Cε (PLCε) increases intracellular Ca 2+ and protein kinase C (PKC) activity in the cardiovascular system in response …

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Structural insights into translocation and tailored synthesis of hyaluronan

Ireneusz Górniak, Zachery Stephens, Satchal K. Erramilli, Tomasz Gawda, Anthony A. Kossiakoff & Jochen Zimmer

Nature Structural & Molecular Biology (2024)

PMID: 39322765 DOI: 10.1038/s41594-024-01389-1

Hyaluronan (HA) is an essential component of the vertebrate extracellular matrix. It is a heteropolysaccharide of N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcA) reaching several megadaltons in healthy tissues. HA is …

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Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Yaqi Liu, Chelsea M Brown, Nuno Borges, Rodrigo N Nobre, Satchal Erramilli, Meagan Belcher Dufrisne, Brian Kloss, Sabrina Giacometti, Ana M Esteves, Cristina G Timoteo, Piotr Tokarz, Rosemary Cater, Yasu S Morita, Anthony A Kossiakoff, Helena Santos, Phillip J Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 18

PMID: 39345506 PMCID: PMC11429742 DOI: 10.1101/2024.09.17.613550

Tuberculosis …

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Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Yaqi Liu, Chelsea M. Brown, Satchal Erramilli, Yi-Chia Su, Po-Sen Tseng, Yu-Jen Wang, Nam Ha Duong, Piotr Tokarz, Brian Kloss, Cheng-Ruei Han, Hung-Yu Chen, José Rodrigues, Margarida Archer, Todd L. Lowary, Anthony A. Kossiakoff, Phillip J. Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 17

PMID: 39345558 PMCID: PMC11429727 DOI: 10.1101/2024.09.17.613533

The emergence of drug-resistant …

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Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which contains several minor vault components (MVCs) and …

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Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

Science Direct Structure 8 October 2024

PMID: 39383874 DOI:10.1016/j.str.2024.09.015

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE binds selectively to membrane protein receptors called claudins on …

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Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial …

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