Kossiakoff Lab – Page 2 – University of Chicago

Oct 14 2024

Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Filed under publications

Yaqi Liu, Chelsea M. Brown, Satchal Erramilli, Yi-Chia Su, Po-Sen Tseng, Yu-Jen Wang, Nam Ha Duong, Piotr Tokarz, Brian Kloss, Cheng-Ruei Han, Hung-Yu Chen, José Rodrigues, Margarida Archer, Todd L. Lowary, Anthony A. Kossiakoff, Phillip J. Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 17

PMID: 39345558 PMCID: PMC11429727 DOI: 10.1101/2024.09.17.613533

The emergence of drug-resistant …

cryo-EM, cryogenic electron microscopy, drug-resistant strains, Fab, Protein structure, Structural biology

Aug 12 2024

Engineered Protein-G variants for plug-and-play applications

Filed under publications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

bioRxiv 2024 August 08.

PMID: 39211271 PMCID: PMC11361062 DOI: 10.1101/2024.08.06.606809

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are …

Bispecific antibodies, Fabs

Aug 07 2024

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Filed under publications

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which contains several minor vault components (MVCs) and …

cryo-EM, cryogenic electron microscopy, Phage display, Protein structure, sAB

Aug 07 2024

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Filed under publications

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

Science Direct Structure 8 October 2024

PMID: 39383874 DOI:10.1016/j.str.2024.09.015

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE binds selectively to membrane protein receptors called claudins on …

cryogenic electron microscopy, Protein structure, X-ray crystallography

Aug 07 2024

Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Filed under publications

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial …

Cryoelectron microscopy, cryogenic electron microscopy, Kinetics, Protein structure, synthetic antibody fragments, X-ray crystallography

Jun 20 2024

Development of Luminescent Biosensors for Calprotectin

Filed under publications

Tong Lan, Tomasz Slezak, Jinyue Pu, Julia Zinkus-Boltz, Sarbani Adhikari, Joel R. Pekow, Vibha Taneja, Joaquin Zuniga, Itzel A. Gómez-García, Nora Regino-Zamarripa, Mushtaq Ahmed, Shabaana A. Khader, David T. Rubin, Anthony A. Kossiakoff, and Bryan C. Dickinson

PMID: 38843544 DOI: 10.1021/acschembio.4c00265

Calprotectin, a metal …

Assays, Bioluminescent probes, Fab, Luminescence, Phage display, Sensors, Serum

Jun 20 2024

The Molecular Basis of Human ALKBH3 Mediated RNA N1-methyladenosine (m1A) Demethylation

Filed under publications

Lin Zhang, Hong-Chao Duan, Marcin Paduch, Jingyan Hu, Chi Zhang, Yajuan Mu, Houwen Lin, Chuan He, Anthony A. Kossiakoff, Guifang Jia, Liang Zhang

Zitierweise: Angew. Chem. Int. Ed. 2024, 63, e202313900

PMID: 38158383 DOI: 10.1002/ange.202313900

N1-methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, and the distribution and dynamics of the modification play key epitranscriptomic roles …

Antibody, Fab, Phage display, Post transcription, Structural biology, X-ray crystallography

Apr 03 2024

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Filed under publications

Hongtao Liu, Dariusz Zakrzewicz, Kamil Nosol, Rossitza N Irobalieva, Somnath Mukherjee, Rose Bang-Sørensen, Nora Goldmann, Sebastian Kunz, Lorenzo Rossi, Anthony A Kossiakoff, Stephan Urban, Dieter Glebe, Joachim Geyer, Kaspar P Locher

Nature Communications volume 15, Article number: 2476 (2024)

PMID: 38509088 PMCID: PMC10954734 DOI: 10.1038/s41467-024-46706-w

Cellular entry of the hepatitis B and D viruses (HBV/HDV) …

Antibody, antiviral, cryo-EM, cryogenic electron microscopy, drug, Fab, hepatitis, Phage display, Protein structure, virus

Apr 03 2024

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Filed under publications

Szymon P. Kordon, Kristina Cechova, Sumit J. Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A. Kossiakoff, Reza Vafabakhsh, and Demet Araç

bioRxiv 2024 Feb 26

PMID: 38464178 DOI: 10.1101/2024.02.25.581807

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain extracellular regions (ECR) containing a conserved GAIN …

Antibody, cell-adhesion molecules, cryo-EM, cryogenic electron microscopy, Fab, Phage display

Nov 21 2023

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

Filed under publications

Heather A Bruce, Alexander U Singer, Ekaterina V Filippova, Levi L Blazer, Jarrett J Adams, Leonie Enderle, Moshe Ben-David, Elizabeth H Radley, Daniel Y L Mao, Victor Pau, Stephen Orlicky, Frank Sicheri, Igor Kourinov, Shane Atwell, Anthony A Kossiakoff, Sachdev S Sidhu

Protein Sci. 2023 Nov 9:e4824.

PMID: 37945533 DOI: 10.1002/pro.4824

The atomic-resolution structural information …

antibody fragments, antibody library, crystal lattice contacts, crystallization platform, protein engineering, surface entropy reduction, X-ray crystallography

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Structural insights into translocation and tailored synthesis of hyaluronan

Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Engineered Protein-G variants for plug-and-play applications

Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Engineered Protein-G variants for plug-and-play applications

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Development of Luminescent Biosensors for Calprotectin

The Molecular Basis of Human ALKBH3 Mediated RNA N1-methyladenosine (m1A) Demethylation

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

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Automated antibody phage display selection and screening system

Recent News

2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies