Feb 08 2009
Mandal K, Pentelute BL, Tereshko V, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2009 Feb;131(4):1362-3
PMID: 19133782
Abstract
Racemic protein crystallography, enabled by total chemical synthesis, has allowed us to determine the X-ray structure of native scorpion toxin BmBKTx1; direct methods were used for phase determination. This is the first example of a protein …
Feb 08 2009
Horn JR, Sosnick TR, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Feb;106(8):2559-64
PMID: 19196954
Abstract
The binding transition state (TS) is the rate-limiting step for transient molecular interactions. This important step in the molecular recognition process, however, is largely understood only at a qualitative level. To establish a more quantitative picture of the TS …
Aug 08 2008
Kossiakoff AA, Koide S
Curr. Opin. Struct. Biol. 2008 Aug;18(4):499-506
PMID: 18638552
Abstract
Recent advances in methodologies and design of combinatorial library selection have enabled comprehensive characterization of sequence space for protein-protein interaction interfaces and generation of fully synthetic binding interfaces. By exhaustively introducing and quantitatively analyzing mutations in natural interfaces, new insights into their …
Jul 08 2008
Tereshko V, Uysal S, Koide A, Margalef K, Koide S, Kossiakoff AA
Protein Sci. 2008 Jul;17(7):1175-87
PMID: 18445622
Abstract
A crystallization chaperone is an auxiliary protein that binds to a target of interest, enhances and modulates crystal packing, and provides high-quality phasing information. We critically evaluated the effectiveness of a camelid single-domain antibody (V(H)H) as …
Jul 08 2008
Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2008 Jul;130(30):9695-701
PMID: 18598029
Abstract
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the …
May 08 2008
Qiu Y, Zhang R, Binkowski TA, Tereshko V, Joachimiak A, Kossiakoff A
Proteins 2008 May;71(2):525-33
PMID: 18175314
Abstract
The DmsD protein is necessary for the biogenesis of dimethyl sulphoxide (DMSO) reductase in many prokaryotes. It performs a critical chaperone function initiated through its binding to the twin-arginine signal peptide of DmsA, the catalytic subunit of …
Jan 08 2008
Ye JD, Tereshko V, Frederiksen JK, Koide A, Fellouse FA, Sidhu SS, Koide S, Kossiakoff AA, Piccirilli JA
Proc. Natl. Acad. Sci. U.S.A. 2008 Jan;105(1):82-7
PMID: 18162543
Abstract
Antibodies that bind protein antigens are indispensable in biochemical research and modern medicine. However, knowledge of RNA-binding antibodies and their application in the ever-growing RNA field is …
Nov 08 2007
Fellouse FA, Esaki K, Birtalan S, Raptis D, Cancasci VJ, Koide A, Jhurani P, Vasser M, Wiesmann C, Kossiakoff AA, Koide S, Sidhu SS
J. Mol. Biol. 2007 Nov;373(4):924-40
PMID: 17825836
Abstract
We have previously established a minimalist approach to antibody engineering by using a phage-displayed framework to support complementarity determining region (CDR) diversity restricted …
Nov 08 2007
Koide A, Tereshko V, Uysal S, Margalef K, Kossiakoff AA, Koide S
J. Mol. Biol. 2007 Nov;373(4):941-53
PMID: 17888451
Abstract
A major architectural class in engineered binding proteins (“antibody mimics”) involves the presentation of recognition loops off a single-domain scaffold. This class of binding proteins, both natural and synthetic, has a strong tendency to bind …
Jun 08 2007
Sidhu SS, Kossiakoff AA
Curr Opin Chem Biol 2007 Jun;11(3):347-54
PMID: 17500026
Abstract
Combinatorial libraries with restricted diversity can be used to rapidly map binding energetics across protein interfaces. Shotgun scanning strategies have been used for alanine scanning and for alternative mutagenesis schemes that provide high-resolution functional views of binding interfaces. In addition, synthetic antibodies …