Jul 08 2008
Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2008 Jul;130(30):9695-701
PMID: 18598029
Abstract
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the …
May 08 2008
Qiu Y, Zhang R, Binkowski TA, Tereshko V, Joachimiak A, Kossiakoff A
Proteins 2008 May;71(2):525-33
PMID: 18175314
Abstract
The DmsD protein is necessary for the biogenesis of dimethyl sulphoxide (DMSO) reductase in many prokaryotes. It performs a critical chaperone function initiated through its binding to the twin-arginine signal peptide of DmsA, the catalytic subunit of …
Jan 08 2008
Ye JD, Tereshko V, Frederiksen JK, Koide A, Fellouse FA, Sidhu SS, Koide S, Kossiakoff AA, Piccirilli JA
Proc. Natl. Acad. Sci. U.S.A. 2008 Jan;105(1):82-7
PMID: 18162543
Abstract
Antibodies that bind protein antigens are indispensable in biochemical research and modern medicine. However, knowledge of RNA-binding antibodies and their application in the ever-growing RNA field is …
Nov 08 2007
Fellouse FA, Esaki K, Birtalan S, Raptis D, Cancasci VJ, Koide A, Jhurani P, Vasser M, Wiesmann C, Kossiakoff AA, Koide S, Sidhu SS
J. Mol. Biol. 2007 Nov;373(4):924-40
PMID: 17825836
Abstract
We have previously established a minimalist approach to antibody engineering by using a phage-displayed framework to support complementarity determining region (CDR) diversity restricted …
Nov 08 2007
Koide A, Tereshko V, Uysal S, Margalef K, Kossiakoff AA, Koide S
J. Mol. Biol. 2007 Nov;373(4):941-53
PMID: 17888451
Abstract
A major architectural class in engineered binding proteins (“antibody mimics”) involves the presentation of recognition loops off a single-domain scaffold. This class of binding proteins, both natural and synthetic, has a strong tendency to bind …
Jun 08 2007
Sidhu SS, Kossiakoff AA
Curr Opin Chem Biol 2007 Jun;11(3):347-54
PMID: 17500026
Abstract
Combinatorial libraries with restricted diversity can be used to rapidly map binding energetics across protein interfaces. Shotgun scanning strategies have been used for alanine scanning and for alternative mutagenesis schemes that provide high-resolution functional views of binding interfaces. In addition, synthetic antibodies …
Dec 08 2006
Gerdts CJ, Tereshko V, Yadav MK, Dementieva I, Collart F, Joachimiak A, Stevens RC, Kuhn P, Kossiakoff A, Ismagilov RF
Angew. Chem. Int. Ed. Engl. 2006 Dec;45(48):8156-60
PMID: 17099920
Abstract
Aug 08 2006
Pál G, Kouadio JL, Artis DR, Kossiakoff AA, Sidhu SS
J. Biol. Chem. 2006 Aug;281(31):22378-85
PMID: 16762925
Abstract
A novel, quantitative saturation (QS) scanning strategy was developed to obtain a comprehensive data base of the structural and functional effects of all possible mutations across a large protein-protein interface. The QS scan approach was applied to …
Jul 08 2006
Horn JR, Kraybill B, Petro EJ, Coales SJ, Morrow JA, Hamuro Y, Kossiakoff AA
Biochemistry 2006 Jul;45(28):8488-98
PMID: 16834322
Abstract
It is generally accepted that protein and solvation dynamics play fundamental roles in the mechanisms of protein-protein binding; however, assessing their contribution meaningfully has not been straightforward. Here, hydrogen/deuterium exchange mass spectrometry (H/D-Ex) was employed …
May 08 2006
Walsh ST, Kossiakoff AA
J. Mol. Biol. 2006 May;358(3):773-84
PMID: 16546209
Abstract
In primates, placental lactogen (PL) is a pituitary hormone with fundamental roles during pregnancy involving fetal growth, metabolism, and stimulating lactation in the mother. Human placental lactogen (hPL) is highly conserved with human growth hormone (hGH) and both hormones bind to the hPRLR …