Nov 08 2007
Koide A, Tereshko V, Uysal S, Margalef K, Kossiakoff AA, Koide S
J. Mol. Biol. 2007 Nov;373(4):941-53
PMID: 17888451
Abstract
A major architectural class in engineered binding proteins (“antibody mimics”) involves the presentation of recognition loops off a single-domain scaffold. This class of binding proteins, both natural and synthetic, has a strong tendency to bind …
Jun 08 2007
Sidhu SS, Kossiakoff AA
Curr Opin Chem Biol 2007 Jun;11(3):347-54
PMID: 17500026
Abstract
Combinatorial libraries with restricted diversity can be used to rapidly map binding energetics across protein interfaces. Shotgun scanning strategies have been used for alanine scanning and for alternative mutagenesis schemes that provide high-resolution functional views of binding interfaces. In addition, synthetic antibodies …
Aug 08 2006
Pál G, Kouadio JL, Artis DR, Kossiakoff AA, Sidhu SS
J. Biol. Chem. 2006 Aug;281(31):22378-85
PMID: 16762925
Abstract
A novel, quantitative saturation (QS) scanning strategy was developed to obtain a comprehensive data base of the structural and functional effects of all possible mutations across a large protein-protein interface. The QS scan approach was applied to …
Jul 08 2006
Horn JR, Kraybill B, Petro EJ, Coales SJ, Morrow JA, Hamuro Y, Kossiakoff AA
Biochemistry 2006 Jul;45(28):8488-98
PMID: 16834322
Abstract
It is generally accepted that protein and solvation dynamics play fundamental roles in the mechanisms of protein-protein binding; however, assessing their contribution meaningfully has not been straightforward. Here, hydrogen/deuterium exchange mass spectrometry (H/D-Ex) was employed …
May 08 2006
Walsh ST, Kossiakoff AA
J. Mol. Biol. 2006 May;358(3):773-84
PMID: 16546209
Abstract
In primates, placental lactogen (PL) is a pituitary hormone with fundamental roles during pregnancy involving fetal growth, metabolism, and stimulating lactation in the mother. Human placental lactogen (hPL) is highly conserved with human growth hormone (hGH) and both hormones bind to the hPRLR …
Mar 08 2006
Bang D, Gribenko AV, Tereshko V, Kossiakoff AA, Kent SB, Makhatadze GI
Nat. Chem. Biol. 2006 Mar;2(3):139-43
PMID: 16446709
Abstract
The alpha-helix is a fundamental protein structural motif and is frequently terminated by a glycine residue. Explanations for the predominance of glycine at the C-cap terminal portions of alpha-helices have invoked uniquely favorable energetics of …
Mar 08 2006
Rizvi SA, Tereshko V, Kossiakoff AA, Kozmin SA
J. Am. Chem. Soc. 2006 Mar;128(12):3882-3
PMID: 16551075
Abstract
Bistramide A is a highly potent antiproliferative marine natural product from Lissoclinum bistratum. We have previously established actin as the primary cellular receptor of bistramide A. We report herein the X-ray structure of bistramide A bound to monomeric …
Jan 08 2006
Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A
Proteins 2006 Jan;62(1):8-16
PMID: 16287087
Abstract
The structure of Aq_328, an uncharacterized protein from hyperthermophilic bacteria Aquifex aeolicus, has been determined to 1.9 A by using multi-wavelength anomalous diffraction (MAD) phasing. Although the amino acid sequence analysis shows that …
Sep 08 2005
Pál G, Fong SY, Kossiakoff AA, Sidhu SS
Protein Sci. 2005 Sep;14(9):2405-13
PMID: 16131663
Abstract
Combinatorial shotgun scanning mutagenesis was used to analyze two large, related protein binding sites to assess the specificity and importance of individual side chain contributions to binding affinity. The strategy allowed for cost-effective generation of a plethora of functional data. …