Mar 08 2006
Bang D, Gribenko AV, Tereshko V, Kossiakoff AA, Kent SB, Makhatadze GI
Nat. Chem. Biol. 2006 Mar;2(3):139-43
PMID: 16446709
Abstract
The alpha-helix is a fundamental protein structural motif and is frequently terminated by a glycine residue. Explanations for the predominance of glycine at the C-cap terminal portions of alpha-helices have invoked uniquely favorable energetics of …
Mar 08 2006
Rizvi SA, Tereshko V, Kossiakoff AA, Kozmin SA
J. Am. Chem. Soc. 2006 Mar;128(12):3882-3
PMID: 16551075
Abstract
Bistramide A is a highly potent antiproliferative marine natural product from Lissoclinum bistratum. We have previously established actin as the primary cellular receptor of bistramide A. We report herein the X-ray structure of bistramide A bound to monomeric …
Jan 08 2006
Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A
Proteins 2006 Jan;62(1):8-16
PMID: 16287087
Abstract
The structure of Aq_328, an uncharacterized protein from hyperthermophilic bacteria Aquifex aeolicus, has been determined to 1.9 A by using multi-wavelength anomalous diffraction (MAD) phasing. Although the amino acid sequence analysis shows that …
Sep 08 2005
Pál G, Fong SY, Kossiakoff AA, Sidhu SS
Protein Sci. 2005 Sep;14(9):2405-13
PMID: 16131663
Abstract
Combinatorial shotgun scanning mutagenesis was used to analyze two large, related protein binding sites to assess the specificity and importance of individual side chain contributions to binding affinity. The strategy allowed for cost-effective generation of a plethora of functional data. …
Jul 08 2005
Kouadio JL, Horn JR, Pal G, Kossiakoff AA
J. Biol. Chem. 2005 Jul;280(27):25524-32
PMID: 15857837
Abstract
The high affinity binding site (Site1) of the human growth hormone (hGH) binds to its cognate receptor (hGHR) via a concave surface patch containing about 35 residues. Using 167 sequences from a shotgun alanine scanning analysis of Site1, we …
Apr 08 2005
Pál G, Ultsch MH, Clark KP, Currell B, Kossiakoff AA, Sidhu SS
J. Mol. Biol. 2005 Apr;347(3):489-94
PMID: 15755445
Abstract
Combinatorial shotgun alanine-scanning was used to assess intramolecular cooperativity in the high affinity site (site 1) of human growth hormone (hGH) for binding to its receptor. A total of 19 side-chains were analyzed and statistically …
Jan 08 2005
Gopal B, Madan LL, Betz SF, Kossiakoff AA
Biochemistry 2005 Jan;44(1):193-201
PMID: 15628860
Abstract
Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with …
Dec 08 2004
Walsh ST, Sylvester JE, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2004 Dec;101(49):17078-83
PMID: 15563602
Abstract
Growth hormone regulates its biological properties via a sequential hormone-induced receptor homodimerization mechanism. Using a mutagenesis-scanning analysis of 81 single and 32 pairwise double mutations, we show that the hormone’s two spatially distal receptor binding sites (Site1 and Site2) …
Jun 08 2004
Kossiakoff AA
Adv. Protein Chem. 2004;68:147-69
PMID: 15500861
Abstract
The pituitary hormones growth hormone (GH), prolactin (PRL) and placental lactogen (PL), are members of an extensive cytokine superfamily of hormones and receptors that share many of the same general structure-function relationships in expressing their biological activities. The biology of the pituitary hormones involves a very …