May 16 2013
Wells JA, Kossiakoff AA
Cell Biology, Volume 344, Issue 6185, pp. 703-704
Abstract
Much of multicellular biology depends on communication through extracellular signaling molecules that bind to membrane receptors and trigger intracellular signaling events and cell behaviors. One of the first paradigm systems for understanding how polypeptide hormones bind and activate their receptors was …
Apr 21 2013
Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ
Nature 2013 May;497(7447):137-41
PMID: 23604254
Abstract
The functions of G-protein-coupled receptors (GPCRs) are primarily mediated and modulated by three …
Mar 15 2013
Paduch M, Koide A, Uysal S, Rizk SS, Koide S, Kossiakoff AA
Methods 2013 Mar;60(1):3-14
PMID: 23280336
Abstract
A set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties. Exquisite control of antigen and conditions during the phage display selection process …
Jan 18 2012
Rizk SS, Misiura A, Paduch M, Kossiakoff AA
Bioconjug. Chem. 2012 Jan;23(1):42-6
PMID: 22175275
Abstract
The use of proteins or nucleic acids as therapeutic agents has been severely hampered by their intrinsic inability to cross the cell membrane. Moreover, common techniques for driving the delivery of macromolecules lack the ability to distinguish between healthy and …
Jul 08 2011
Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E
Proc. Natl. Acad. Sci. U.S.A. 2011 Jul;108(29):11896-9
PMID: 21730186
Abstract
Using a constitutively active channel mutant, we solved the structure of full-length KcsA in the open conformation at 3.9 Å. The structure reveals that the activation gate expands about 20 Å, exerting a strain on …
Apr 08 2011
Rizk SS, Paduch M, Heithaus JH, Duguid EM, Sandstrom A, Kossiakoff AA
Nat. Struct. Mol. Biol. 2011 Apr;18(4):437-42
PMID: 21378967
Abstract
We describe a phage display methodology for engineering synthetic antigen binders (sABs) that recognize either the apo or the ligand-bound conformation of maltose-binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP …
Jan 08 2011
Koldobskaya Y, Duguid EM, Shechner DM, Suslov NB, Ye J, Sidhu SS, Bartel DP, Koide S, Kossiakoff AA, Piccirilli JA
Nat. Struct. Mol. Biol. 2011 Jan;18(1):100-6
PMID: 21151117
Abstract
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic …
Nov 08 2010
Brawley CM, Uysal S, Kossiakoff AA, Rock RS
PLoS ONE 2010 Nov;5(11):e13960
PMID: 21103060
Abstract
BACKGROUND: Eukaryotic cells strictly regulate the structure and assembly of their actin filament networks in response to various stimuli. The actin binding proteins that control filament assembly are therefore attractive targets for those who wish to reorganize actin filaments and …
Mar 08 2010
Kossiakoff AA, Rizk S
Structure 2010 Mar;18(3):275-6
PMID: 20223207
Abstract
Cytokine signaling is triggered by a hormone-induced receptor aggregation process. IL-13 employs an unconventional sequence of steps for assembling its signaling complex to trigger activation and for turning it off. Both these processes involve some unusual molecular recognition features, as discussed by Lupardus et al. …
Jul 08 2009
Rizk SS, Luchniak A, Uysal S, Brawley CM, Rock RS, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Jul;106(27):11011-5
PMID: 19549879
Abstract
We have developed and tested a robust delivery method for the transport of proteins to the cytoplasm of mammalian cells without compromising the integrity of the cell membrane. This receptor-mediated delivery (RMD) technology …