Mar 04 2022
Ahmed Rohaim, Tomasz Slezak, Young Hoon Koh, Lydia Blachowicz, Anthony A Kossiakoff, Benoît Roux
J Gen Physiol. 2022 Apr 4;154(4):e202112965.
PMID: 35234830 DOI: 10.1085/jgp.202112965
Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid structural determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges of routine …
Jan 04 2022
Elena K Davydova
Review Biochemistry (Mosc). 2022 Jan;87(Suppl 1):S146-S110
PMID: 35501993 PMCID: PMC8802281 DOI: 10.1134/S0006297922140127
Functional Protein Engineering became the hallmark of biomolecule manipulation in the new millennium, building on and surpassing the underlying structural DNA manipulation and recombination techniques developed and employed in the last decades of the 20th century. Because of their prominence …
Dec 06 2021
Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher
Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)
PMID: 34782475 DOI: 10.1073/pnas.2115435118
With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …
Oct 24 2021
Kamil Nosol, Rose Bang-Sørensen, Rossitza N Irobalieva, Satchal K Erramilli, Bruno Stieger, Anthony A Kossiakoff, Kaspar P Locher
Proc Natl Acad Sci USA 2021 Aug 17;118(33):e2106702118.
PMID: 34385322 PMCID: PMC8379956 (available on 2022-02-12)
DOI: 10.1073/pnas.2106702118
ABCB4 is expressed in hepatocytes and translocates phosphatidylcholine into bile canaliculi. The mechanism of specific lipid recruitment from the canalicular …
Jul 23 2021
Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher
Science 23 Jul 2021: …
Jul 20 2021
Qiuyan Chen, Manolo Plasencia, Zhuang Li, Somnath Mukherjee, Dhabaleswar Patra, Chun-Liang Chen, Thomas Klose, Xin-Qiu Yao, Anthony A Kossiakoff, Leifu Chang, Philip C Andrews, John J G Tesmer
Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x
PMID: 34262173
G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization1. Although it is unclear how …
Jul 13 2021
Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff, Lei Liu & Minglei Zhao
Nat Chem Biol. 2021 Jul 8. doi: 10.1038/s41589-021-00823-5
PMID: 34239127
Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin via different linkages. Deciphering the cellular ubiquitin …
Jul 06 2021
Leonie Enderle, Karim H Shalaby, Maryna Gorelik, Alexander Weiss, Levi L Blazer, Marcin Paduch, Lia Cardarelli, Anthony Kossiakoff, Jarrett J Adams, Sachdev S Sidhu
MAbs. Jan-Dec 2021;13(1):1933690. doi: 10.1080/19420862.2021.1933690
PMID: 34190031
In order to direct T cells to specific features of solid cancer cells, we engineered a bispecific antibody format, named Dual Antigen T cell …
Jun 16 2021
Rosemary J Cater, Geok Lin Chua, Satchal K Erramilli, James E Keener, Brendon C Choy, Piotr Tokarz, Cheen Fei Chin, Debra Q Y Quek, Brian Kloss, Joseph G Pepe, Giacomo Parisi, Bernice H Wong, Oliver B Clarke, Michael T Marty, Anthony A Kossiakoff, George Khelashvili, David L Silver, Filippo Mancia
Nature volume 595, pages315–319 (2021)
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Jun 04 2021
Gallo E, Kelil A, Haughey M, Cazares-Olivera M, Yates BP, Zhang M, Wang NY, Blazer L, Carderelli L, Adams JJ, Kossiakoff AA, Wells JA, Xie W, Sidhu SS
J Mol Biol. 2021 Jun 4;433(15):167090. doi: 10.1016/j.jmb.2021.167090.
PMID: 34090922
Members of the αv family of integrins regulate activation of transforming growth factor beta (TGFβ) and are …