Welcome to the Kossiakoff Lab website

The Kossiakoff Group’s research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful than traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

Latest Publications

A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy

Arina A; Arauz E; Masoumi E; Warzecha K W; Sääf A; Widło Ł; Slezak T; Zieminska A; Dudek K; Schaefer Z P; Lecka M; Usatyuk S; Weichselbaum R R; Kossiakoff A A

A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy Journal Article

In: Sci Adv, vol. 11, no. 27, pp. eadv4937, 2025, ISSN: 2375-2548.

Abstract | Links

Biophysical basis of tight junction barrier modulation by a pan-claudin-binding molecule

Ogbu C P; de Las Alas M; Mandriota A M; Liu X; Kapoor S; Choudhury J; Ruma Y N; Goodman M C; Sanders C R; Gonen T; Kossiakoff A A; Duffey M E; Vecchio A J

Biophysical basis of tight junction barrier modulation by a pan-claudin-binding molecule Journal Article

In: PNAS Nexus, vol. 4, no. 6, pp. pgaf189, 2025, ISSN: 2752-6542.

Abstract | Links

Conformation-specific synthetic intrabodies modulate mTOR signaling with subcellular spatial resolution

O'Leary K M; Slezak T; Kossiakoff A A

Conformation-specific synthetic intrabodies modulate mTOR signaling with subcellular spatial resolution Journal Article

In: Proc Natl Acad Sci U S A, vol. 122, no. 24, pp. e2424679122, 2025, ISSN: 1091-6490.

Abstract | Links

Effect of phosphorylation barcodes on arrestin binding to a chemokine receptor

Chen Q; Schafer C T; Mukherjee S; Wang K; Gustavsson M; Fuller J R; Tepper K; Lamme T D; Aydin Y; Agrawal P; Terashi G; Yao X; Kihara D; Kossiakoff A A; Handel T M; Tesmer J J G

Effect of phosphorylation barcodes on arrestin binding to a chemokine receptor Journal Article

In: Nature, 2025, ISSN: 1476-4687.

Abstract | Links

Structural basis for nucleolin recognition of MYC promoter G-quadruplex

Chen L; Dickerhoff J; Zheng K; Erramilli S; Feng H; Wu G; Onel B; Chen Y; Wang K; Carver M; Lin C; Sakai S; Wan J; Vinson C; Hurley L; Kossiakoff A A; Deng N; Bai Y; Noinaj N; Yang D

Structural basis for nucleolin recognition of MYC promoter G-quadruplex Journal Article

In: Science, vol. 388, no. 6744, 2025, ISSN: 1095-9203.

Abstract | Links

Mapping the extracellular molecular architecture of the pAg-signaling complex with α-Butyrophilin antibodies

Ramesh A; Roy S; Slezak T; Fuller J; Graves H; Mamedov M R; Marson A; Kossiakoff A A; Adams E J

Mapping the extracellular molecular architecture of the pAg-signaling complex with α-Butyrophilin antibodies Journal Article

In: Sci Rep, vol. 15, no. 1, 2025, ISSN: 2045-2322.

Abstract | Links

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