Kossiakoff Lab – Page 3 – University of Chicago

Jul 05 2022

Quaternary structure independent folding of voltage-gated ion channel pore domain subunits

Filed under publications

Cristina Arrigoni, Marco Lolicato, David Shaya, Ahmed Rohaim, Felix Findeisen, Lam-Kiu Fong, Claire M Colleran, Pawel Dominik, Sangwoo S Kim, Jonathan P Schuermann, William F DeGrado, Michael Grabe, Anthony A Kossiakoff, Daniel L Minor

Nat Struct Mol Biol. 2022 Jun;29(6):537-548.

PMID: 35655098 DOI: 10.1038/s41594-022-00775-x

Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from …

Fabs, Membrane biophysics, Phage display, Quaternary structure, X-ray crystallography

May 13 2022

Synthetic antibody binders detect distinct cellular states of Chromosome Passenger Complex proteins

Filed under publications

Marcin Ura, Somnath Mukherjee, Edyta Marcon, Stefan A Koestler, Anthony A Kossiakoff

J Mol Biol. 2022 Apr 22;167602.

PMID: 35469831 DOI: 10.1016/j.jmb.2022.167602

High-performance affinity reagents are essential tools to enable biologists to profile the cellular location and composition of macromolecular complexes undergoing dynamic reorganization. To support further development of such tools, we have assembled a …

Borealin, INCENP, mitosis, Phage display, Survivin, synthetic antibody binders

Apr 07 2022

Structural basis of lipopolysaccharide maturation by the O-antigen ligase

Filed under publications

Khuram U. Ashraf, Rie Nygaard, Owen N. Vickery, Satchal K. Erramilli, Carmen M. Herrera, Thomas H. McConville, Vasileios I. Petrou, Sabrina I. Giacometti, Meagan Belcher Dufrisne, Kamil Nosol, Allen P. Zinkle, Chris L. B. Graham, Michael Loukeris, Brian Kloss, Karolina Skorupinska-Tudek, Ewa Swiezewska, David I. Roper, Oliver B. Clarke, Anne-Catrin Uhlemann, Anthony A. Kossiakoff, M. …

CmWaaL, cryo-EM, Protein structure, Recombinant Antibody

Mar 07 2022

Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers

Filed under publications

Shion A Lim, Jie Zhou, Alexander J Martinko, Yung-Hua Wang, Ekaterina V Filippova, Veronica Steri, Donghui Wang, Soumya G Remesh, Jia Liu, Byron Hann, Anthony A Kossiakoff, Michael J Evans, Kevin K Leung, James A Wells

J Clin Invest. 2022 Feb 15;132(4):e154604.

PMID: 35166238 PMCID: PMC8843743 DOI: 10.1172/JCI154604

Extracellular proteolysis is frequently dysregulated in disease and can generate …

Antigen, Cancer immunotherapy, Proteases, Therapeutics

Mar 04 2022

Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels

Filed under publications

Ahmed Rohaim, Tomasz Slezak, Young Hoon Koh, Lydia Blachowicz, Anthony A Kossiakoff, Benoît Roux

J Gen Physiol. 2022 Apr 4;154(4):e202112965.

PMID: 35234830 DOI: 10.1085/jgp.202112965

Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid structural determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges of routine …

engineered antibody fragments, synthetic antibody fragment

Dec 06 2021

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Filed under publications

Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)

PMID: 34782475 DOI: 10.1073/pnas.2115435118

With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …

Antibody, cryogenic electron microscopy, membrane protein, nanobody, structure

Oct 24 2021

Structures of ABCB4 provide insight into phosphatidylcholine translocation

Filed under publications

Kamil Nosol, Rose Bang-Sørensen, Rossitza N Irobalieva, Satchal K Erramilli, Bruno Stieger, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci USA 2021 Aug 17;118(33):e2106702118.

PMID: 34385322 PMCID: PMC8379956 (available on 2022-02-12)

DOI: 10.1073/pnas.2106702118

ABCB4 is expressed in hepatocytes and translocates phosphatidylcholine into bile canaliculi. The mechanism of specific lipid recruitment from the canalicular …

ABC transporter, ABCB4, cryo-EM, hepatocyte, membrane transport, phosphatidylcholine, posaconazole

Jul 23 2021

Structure of an AMPK complex in an inactive, ATP-bound state

Filed under publications

Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher

Science 23 Jul 2021: …

AMP, AMPK, ATP

Jul 20 2021

Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1

Filed under publications

Qiuyan Chen, Manolo Plasencia, Zhuang Li, Somnath Mukherjee, Dhabaleswar Patra, Chun-Liang Chen, Thomas Klose, Xin-Qiu Yao, Anthony A Kossiakoff, Leifu Chang, Philip C Andrews, John J G Tesmer

Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x

PMID: 34262173

G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization1. Although it is unclear how …

Jul 13 2021

K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle

Filed under publications

Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff, Lei Liu & Minglei Zhao

Nat Chem Biol. 2021 Jul 8. doi: 10.1038/s41589-021-00823-5

PMID: 34239127

Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin via different linkages. Deciphering the cellular ubiquitin …

Peptide synthesis, Phage display, Proteomics

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Quaternary structure independent folding of voltage-gated ion channel pore domain subunits

Synthetic antibody binders detect distinct cellular states of Chromosome Passenger Complex proteins

Structural basis of lipopolysaccharide maturation by the O-antigen ligase

Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers

Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Structures of ABCB4 provide insight into phosphatidylcholine translocation

Structure of an AMPK complex in an inactive, ATP-bound state

Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1

K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle

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Recent News

2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies