Jan 08 2011
Koldobskaya Y, Duguid EM, Shechner DM, Suslov NB, Ye J, Sidhu SS, Bartel DP, Koide S, Kossiakoff AA, Piccirilli JA
Nat. Struct. Mol. Biol. 2011 Jan;18(1):100-6
PMID: 21151117
Abstract
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic …
Nov 08 2010
Brawley CM, Uysal S, Kossiakoff AA, Rock RS
PLoS ONE 2010 Nov;5(11):e13960
PMID: 21103060
Abstract
BACKGROUND: Eukaryotic cells strictly regulate the structure and assembly of their actin filament networks in response to various stimuli. The actin binding proteins that control filament assembly are therefore attractive targets for those who wish to reorganize actin filaments and …
Mar 08 2010
Kossiakoff AA, Rizk S
Structure 2010 Mar;18(3):275-6
PMID: 20223207
Abstract
Cytokine signaling is triggered by a hormone-induced receptor aggregation process. IL-13 employs an unconventional sequence of steps for assembling its signaling complex to trigger activation and for turning it off. Both these processes involve some unusual molecular recognition features, as discussed by Lupardus et al. …
Jul 08 2009
Rizk SS, Luchniak A, Uysal S, Brawley CM, Rock RS, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Jul;106(27):11011-5
PMID: 19549879
Abstract
We have developed and tested a robust delivery method for the transport of proteins to the cytoplasm of mammalian cells without compromising the integrity of the cell membrane. This receptor-mediated delivery (RMD) technology …
Jul 08 2009
Bang D, Tereshko V, Kossiakoff AA, Kent SB
Mol Biosyst 2009 Jul;5(7):750-6
PMID: 19562114
Abstract
We have used total chemical synthesis to prepare [V15A]crambin-alpha-carboxamide, a unique protein analogue that eliminates a salt bridge between the delta-guanidinium of the Arg(10) side chain and the alpha-carboxylate of Asn(46) at the C-terminus of the polypeptide chain. This salt …
Jun 08 2009
Mandal K, Pentelute BL, Tereshko V, Thammavongsa V, Schneewind O, Kossiakoff AA, Kent SB
Protein Sci. 2009 Jun;18(6):1146-54
PMID: 19472324
Abstract
We describe the use of racemic crystallography to determine the X-ray structure of the natural product plectasin, a potent antimicrobial protein recently isolated from fungus. The protein enantiomers L-plectasin and D-plectasin were prepared by …
Apr 08 2009
Uysal S, Vásquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A
Proc. Natl. Acad. Sci. U.S.A. 2009 Apr;106(16):6644-9
PMID: 19346472
Abstract
KcsA is a proton-activated, voltage-modulated K(+) channel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments …
Feb 08 2009
Mandal K, Pentelute BL, Tereshko V, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2009 Feb;131(4):1362-3
PMID: 19133782
Abstract
Racemic protein crystallography, enabled by total chemical synthesis, has allowed us to determine the X-ray structure of native scorpion toxin BmBKTx1; direct methods were used for phase determination. This is the first example of a protein …
Feb 08 2009
Horn JR, Sosnick TR, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Feb;106(8):2559-64
PMID: 19196954
Abstract
The binding transition state (TS) is the rate-limiting step for transient molecular interactions. This important step in the molecular recognition process, however, is largely understood only at a qualitative level. To establish a more quantitative picture of the TS …
Aug 08 2008
Kossiakoff AA, Koide S
Curr. Opin. Struct. Biol. 2008 Aug;18(4):499-506
PMID: 18638552
Abstract
Recent advances in methodologies and design of combinatorial library selection have enabled comprehensive characterization of sequence space for protein-protein interaction interfaces and generation of fully synthetic binding interfaces. By exhaustively introducing and quantitatively analyzing mutations in natural interfaces, new insights into their …