Judy Luke

Author's posts

The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab

Radwańska MJ, Jaskółowski M, Davydova E, Derewenda U, Miyake T, Engel DA, Kossiakoff AA, Derewenda ZS

Acta Crystallogr D Struct Biol 2018 Jul;74(Pt 7):681-689

PMID: 29968677

Abstract

The vast majority of platforms for the detection of viral or bacterial antigens rely on immunoassays, typically ELISA or sandwich ELISA, that are contingent on the availability of …

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Cryo-EM structure of human rhodopsin bound to an inhibitory G protein

Kang Y, Kuybeda O, de Waal PW, Mukherjee S, Van Eps N, Dutka P, Zhou XE, Bartesaghi A, Erramilli S, Morizumi T, Gu X, Yin Y, Liu P, Jiang Y, Meng X, Zhao G, Melcher K, Ernst OP, Kossiakoff AA, Subramaniam S, Xu HE

Nature 2018 Jun;

PMID: 29899450

Abstract

G-protein-coupled receptors comprise the largest …

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Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ

Elife 2018 Mar;7

PMID: 29596046

Abstract

Insulin degrading enzyme (IDE) plays key roles …

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Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination

Bailey LJ, Sheehy KM, Dominik PK, Liang WG, Rui H, Clark M, Jaskolowski M, Kim Y, Deneka D, Tang WJ, Kossiakoff AA

J. Mol. Biol. 2018 Feb;430(3):337-347

PMID: 29273204

Abstract

Antibody Fab fragments have been exploited with significant success to facilitate the structure determination of challenging macromolecules as crystallization chaperones and as molecular fiducial marks …

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A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction

Mukherjee S, Ura M, Hoey RJ, Kossiakoff AA

J. Mol. Biol. 2015 Aug;427(16):2707-25

PMID: 26159704

Abstract

Reversible, high-affinity immobilization tags are critical tools for myriad biological applications. However, inherent issues are associated with a number of the current methods of immobilization. Particularly, a critical element in phage display sorting is functional immobilization of target proteins. …

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Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin

Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA

J. Mol. Biol. 2015 Jul;427(15):2532-2547

PMID: 26087144

Abstract

Focal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1-LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic …

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Mechanism of activation gating in the full-length KcsA K+ channel

Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E

Proc. Natl. Acad. Sci. U.S.A. 2011 Jul;108(29):11896-9

PMID: 21730186

Abstract

Using a constitutively active channel mutant, we solved the structure of full-length KcsA in the open conformation at 3.9 Å. The structure reveals that the activation gate expands about 20 Å, exerting a strain on …

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Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins

Rizk SS, Paduch M, Heithaus JH, Duguid EM, Sandstrom A, Kossiakoff AA

Nat. Struct. Mol. Biol. 2011 Apr;18(4):437-42

PMID: 21378967

Abstract

We describe a phage display methodology for engineering synthetic antigen binders (sABs) that recognize either the apo or the ligand-bound conformation of maltose-binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP …

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A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination

Koldobskaya Y, Duguid EM, Shechner DM, Suslov NB, Ye J, Sidhu SS, Bartel DP, Koide S, Kossiakoff AA, Piccirilli JA

Nat. Struct. Mol. Biol. 2011 Jan;18(1):100-6

PMID: 21151117

Abstract

RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic …

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Characterization of engineered actin binding proteins that control filament assembly and structure

Brawley CM, Uysal S, Kossiakoff AA, Rock RS

PLoS ONE 2010 Nov;5(11):e13960

PMID: 21103060

Abstract

BACKGROUND: Eukaryotic cells strictly regulate the structure and assembly of their actin filament networks in response to various stimuli. The actin binding proteins that control filament assembly are therefore attractive targets for those who wish to reorganize actin filaments and …

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