Tag: membrane protein

Aug 08 2023

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Filed under publications

Susan A Leonhardt, Michael D Purdy, Jonathan R Grover, Ziwei Yang, Sandra Poulos, William E McIntire, Elizabeth A Tatham, Satchal K Erramilli, Kamil Nosol, Kin Kui Lai, Shilei Ding, Maolin Lu, Pradeep D Uchil, Andrés Finzi, Alan Rein, Anthony A Kossiakoff, Walther Mothes, Mark Yeager

Nature Communications volume 14, Article number: 4368 (2023)

PMID: 37474505 …

Antibody, cryo-EM, cryogenic electron microscopy, Fab, membrane protein, Phage display, Protein structure

Aug 03 2023

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Filed under publications

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2023 Jun 12, 2023

PMID: 37398044 PMCID: PMC10312657 DOI: 10.1101/2023.06.12.544689

Claudins are a family of ∼25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. Humans have 27 subtypes, which homo- and hetero-oligomerize to …

cryo-EM, cryogenic electron microscopy, membrane protein, Phage display, Protein structure

Jul 24 2023

Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Filed under publications

Satchal K. Erramilli, Pawel K. Dominik, Dawid Deneka , Piotr Tokarz , Sangwoo S. Kim, Bharat G. Reddy, Blazej M. Skrobek , Olivier Dalmas , Eduardo Perozo, Anthony A. Kossiakoff

J Mol Biol. 2023

PMD: 37394032 PMID: 37394032 DOI: 10.1016/j.jmb.2023.168192

CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg2+, and highly asymmetric …

Antibody, CorA, ion channel, membrane protein, Phage display, synthetic antibodies, X-ray crystallography

Apr 03 2023

Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Filed under publications

Karthik Srinivasan, Satchal K. Erramilli, Srinivas Chakravarthy, Adrian Gonzalez, Anthony Kossiakoff, Nicholas Noinaj

Structure. 2023 Mar 17; S0969-2126(23)00078-3.

PMID: 36977410 DOI: 10.1016/j.str.2023.03.002

Roughly 95% of the proteins that make up the chloroplast must be imported from the cytoplasm. The machinery responsible for the translocation of these cargo proteins is called the translocon at the outer …

chloroplast, cryo-EM, membrane protein, Phage display, plants, plastid, protein translocation, X-ray crystallography

Dec 06 2021

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Filed under publications

Joël S Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A Kossiakoff, Kaspar P Locher

Proc Natl Acad Sci U S A. 2021 Nov 23;118(47)

PMID: 34782475 DOI: 10.1073/pnas.2115435118

With conformation-specific nanobodies being used for a wide range of structural, biochemical, and cell biological applications, there is a …

Antibody, cryogenic electron microscopy, membrane protein, nanobody, structure

2023 NAS Election
May 3, 2023
News from the National Academy of Sciences Newly elected member Anthony A. Kossiakoff; Professor, Department of Biochemistry and Molecular Biology, The University of Chicago Three University of Chicago faculty members elected to the National Academy of Sciences in 2023
Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies
March 1, 2021
The Recombinant Antibody Network (RAN), a consortium comprising research groups from UC San Francisco (UCSF), the University of Chicago, and the University of Toronto have expanded a previous research collaboration with Bristol Myers Squibb, aimed to create and develop high-performance recombinant antibodies against diverse targets in human cells. The RAN was founded in 2012 by … Continue reading
Patent of the Week: Improving the Performance of Antibody Fragments
July 6, 2020
Patent-of-the-week-improving-the-performance-of-antibody-fragments Antibody fragments, including Fabs, which bind to immune response-triggering substances, or antigens, have demonstrated utility in various research and diagnostic applications. However, their structure limits their use, so researchers have engineered a protein that can be tethered to Fabs to expand their functionality. The inventors, including Anthony Kossiakoff, a professor of biochemistry and molecular … Continue reading
Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner
January 2, 2020
The Christian B. Anfinsen Award- Sponsored by The Protein Society, recognizes technological achievement or significant methodological advances in the field of protein science. The recipient of this award in 2019 is Professor Anthony Kossiakoff (University of Chicago). Professor Kossiakoff’s achievements have had broad and sustained impact through the development of innovative technologies and major discoveries … Continue reading
Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies
December 11, 2019
Absolute Antibody Ltd., an industry-leading provider of recombinant antibody products and services, has announced a partnership with the Recombinant Antibody Network (RAN), a consortium of three expert centers at the University of Chicago, University of Toronto, and UC San Francisco (UCSF) with a common goal to generate recombinant antibodies at a proteome-wide scale.

Tag: membrane protein

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA

Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

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2023 NAS Election

Recombinant Antibody Network Partners with Bristol Myers Squibb to Develop Novel Therapies

Patent of the Week: Improving the Performance of Antibody Fragments

Anthony Kossiakoff, University of Chicago 2019 Christian B. Anfinsen Award Winner

Absolute Antibody Partners with the Recombinant Antibody Network to Facilitate Access to Engineered Recombinant Antibodies