Kossiakoff Lab

University of Chicago

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Generating Conformation and Complex-Specific Synthetic Antibodies

Research Area: Uncategorized Year: 2017
Type of Publication: In Book
  • Marcin Paduch
  • Anthony A. Kossiakoff
Editor: Tiller, Thomas
Pages: 93-119
Publisher: Springer New York Address: New York, NY
ISBN: 978-1-4939-6857-2
Phage display is commonly used to identify and isolate binders from large combinatorial libraries. Here we present phage selection protocols enabling generation of synthetic antibodies capable of recognizing multiprotein complexes and conformational states. The procedure describes stages of the experiment design, optimization, and screening, as well as provides the framework for building downstream assays with an end goal of isolating bioactive antibodies for future therapeutic use. The methods described are also applicable to screening directly on cells and can be ported to other in vitro directed evolution systems utilizing non-immunoglobulin scaffolds.
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