Jul 08 2009
Bang D, Tereshko V, Kossiakoff AA, Kent SB
Mol Biosyst 2009 Jul;5(7):750-6
PMID: 19562114
Abstract
We have used total chemical synthesis to prepare [V15A]crambin-alpha-carboxamide, a unique protein analogue that eliminates a salt bridge between the delta-guanidinium of the Arg(10) side chain and the alpha-carboxylate of Asn(46) at the C-terminus of the polypeptide chain. This salt …
Jun 08 2009
Mandal K, Pentelute BL, Tereshko V, Thammavongsa V, Schneewind O, Kossiakoff AA, Kent SB
Protein Sci. 2009 Jun;18(6):1146-54
PMID: 19472324
Abstract
We describe the use of racemic crystallography to determine the X-ray structure of the natural product plectasin, a potent antimicrobial protein recently isolated from fungus. The protein enantiomers L-plectasin and D-plectasin were prepared by …
Apr 08 2009
Uysal S, Vásquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A
Proc. Natl. Acad. Sci. U.S.A. 2009 Apr;106(16):6644-9
PMID: 19346472
Abstract
KcsA is a proton-activated, voltage-modulated K(+) channel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments …
Feb 08 2009
Mandal K, Pentelute BL, Tereshko V, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2009 Feb;131(4):1362-3
PMID: 19133782
Abstract
Racemic protein crystallography, enabled by total chemical synthesis, has allowed us to determine the X-ray structure of native scorpion toxin BmBKTx1; direct methods were used for phase determination. This is the first example of a protein …
Feb 08 2009
Horn JR, Sosnick TR, Kossiakoff AA
Proc. Natl. Acad. Sci. U.S.A. 2009 Feb;106(8):2559-64
PMID: 19196954
Abstract
The binding transition state (TS) is the rate-limiting step for transient molecular interactions. This important step in the molecular recognition process, however, is largely understood only at a qualitative level. To establish a more quantitative picture of the TS …
Aug 08 2008
Kossiakoff AA, Koide S
Curr. Opin. Struct. Biol. 2008 Aug;18(4):499-506
PMID: 18638552
Abstract
Recent advances in methodologies and design of combinatorial library selection have enabled comprehensive characterization of sequence space for protein-protein interaction interfaces and generation of fully synthetic binding interfaces. By exhaustively introducing and quantitatively analyzing mutations in natural interfaces, new insights into their …
Jul 08 2008
Tereshko V, Uysal S, Koide A, Margalef K, Koide S, Kossiakoff AA
Protein Sci. 2008 Jul;17(7):1175-87
PMID: 18445622
Abstract
A crystallization chaperone is an auxiliary protein that binds to a target of interest, enhances and modulates crystal packing, and provides high-quality phasing information. We critically evaluated the effectiveness of a camelid single-domain antibody (V(H)H) as …
Jul 08 2008
Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB
J. Am. Chem. Soc. 2008 Jul;130(30):9695-701
PMID: 18598029
Abstract
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the …
May 08 2008
Qiu Y, Zhang R, Binkowski TA, Tereshko V, Joachimiak A, Kossiakoff A
Proteins 2008 May;71(2):525-33
PMID: 18175314
Abstract
The DmsD protein is necessary for the biogenesis of dimethyl sulphoxide (DMSO) reductase in many prokaryotes. It performs a critical chaperone function initiated through its binding to the twin-arginine signal peptide of DmsA, the catalytic subunit of …
Jan 08 2008
Ye JD, Tereshko V, Frederiksen JK, Koide A, Fellouse FA, Sidhu SS, Koide S, Kossiakoff AA, Piccirilli JA
Proc. Natl. Acad. Sci. U.S.A. 2008 Jan;105(1):82-7
PMID: 18162543
Abstract
Antibodies that bind protein antigens are indispensable in biochemical research and modern medicine. However, knowledge of RNA-binding antibodies and their application in the ever-growing RNA field is …